The BOS loci of Arabidopsis are required for resistance to Botrytis cinerea infection.
The BOS loci of Arabidopsis are required for resistance to Botrytis cinerea infection.
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DOI：
10.1111/j.1365-313x.2004.02232.x
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发表时间：
2004-11
期刊：
影响因子：
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通讯作者：
P. Veronese;X. Chen;B. Bluhm;J. Salmeron;R. Dietrich;T. Mengiste
中科院分区：
文献类型：
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作者：
P. Veronese;X. Chen;B. Bluhm;J. Salmeron;R. Dietrich;T. Mengiste
分离到3个对灰葡萄孢菌敏感的突变体bos 2、bos 3和bos 4，它们定义了拟南芥对灰葡萄孢菌的抗性所需的独立和新的遗传位点。bos 2突变体对B敏感。灰霉病菌，但保留野生型水平的其他病原体测试的阻力，表明在响应途径的缺陷更具体到B。灰色区域。bos 3和bos 4突变体也表现出增加的易感性，另一个necrotrophic病原体，芸苔链格孢，这表明这些位点的抗性更广泛的作用。bos 4表现出最宽的抗性范围，对假单胞菌pv.番茄有趣的是，bos 3比野生型植物对活体营养型病原体寄生霜霉（Peronospora parasitica）和细菌病原体黄瓜霜霉致病变种（P.番茄致病相关基因1（PR-1）是水杨酸（SA）依赖性抗性途径的分子标记物，其在bos 2中显示野生型表达模式，而在bos 3中，该基因以升高的水平表达，包括组成型和响应病原体挑战。在bos 4植物中，PR-1表达与野生型相比响应于B而降低。cinerea和SA.在bos 3中，突变体对B最敏感。灰霉病和PR-1的表达最高，SA的去除导致PR-1表达降低，但对B没有变化。灰膜反应与野生型植物相比，植物防御素基因PDF 1 -2的表达在bos突变体中普遍较低，其中bos 3的表达降低尤其强烈。植物抗毒素Camalexin的产生是另一种充分表征的植物防御反应。响应于B，bos 2和bos 4突变体积累降低水平的Camalexin，而bos 3积累比野生型植物显著更高水平的Camalexin。灰色区域。BOS 2、BOS 3和BOS 4基因座可能影响山茶素水平以及对乙烯和茉莉酸盐的反应性。这三个新的突变体似乎通过独立于先前描述的BOS 1基因的机制介导疾病反应。基于bos突变体表型的差异，似乎它们影响防御反应途径的不同点。
Three Botrytis-susceptible mutants bos2, bos3, and bos4 which define independent and novel genetic loci required for Arabidopsis resistance to Botrytis cinerea were isolated. The bos2 mutant is susceptible to B. cinerea but retains wild-type levels of resistance to other pathogens tested, indicative of a defect in a response pathway more specific to B. cinerea. The bos3 and bos4 mutants also show increased susceptibility to Alternaria brassicicola, another necrotrophic pathogen, suggesting a broader role for these loci in resistance. bos4 shows the broadest range of effects on resistance, being more susceptible to avirulent strain of Pseudomonas syringae pv. tomato. Interestingly, bos3 is more resistant than wild-type plants to virulent strains of the biotrophic pathogen Peronospora parasitica and the bacterial pathogen P. syringae pv. tomato. The Pathogenesis Related gene 1 (PR-1), a molecular marker of the salicylic acid (SA)-dependent resistance pathway, shows a wild-type pattern of expression in bos2, while in bos3 this gene was expressed at elevated levels, both constitutively and in response to pathogen challenge. In bos4 plants, PR-1 expression was reduced compared with wild type in response to B. cinerea and SA. In bos3, the mutant most susceptible to B. cinerea and with the highest expression of PR-1, removal of SA resulted in reduced PR-1 expression but no change to the B. cinerea response. Expression of the plant defensin gene PDF1-2 was generally lower in bos mutants compared with wild-type plants, with a particularly strong reduction in bos3. Production of the phytoalexin camalexin is another well-characterized plant defense response. The bos2 and bos4 mutants accumulate reduced levels of camalexin whereas bos3 accumulates significantly higher levels of camalexin than wild-type plants in response to B. cinerea. The BOS2, BOS3, and BOS4 loci may affect camalexin levels and responsiveness to ethylene and jasmonate. The three new mutants appear to mediate disease responses through mechanisms independent of the previously described BOS1 gene. Based on the differences in the phenotypes of the bos mutants, it appears that they affect different points in defense response pathways.