Molecular analysis of Malassezia microflora from patients with pityriasis versicolor
Molecular analysis of Malassezia microflora from patients with pityriasis versicolor
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DOI：
10.1007/s11046-005-0149-4
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发表时间：
2006-02-01
期刊：
影响因子：
5.5
通讯作者：
Sugita, T
中科院分区：
文献类型：
--
作者：
Morishita, N;Sei, Y;Sugita, T
背景：花斑癣是由马拉色菌引起的皮肤角质层浅表感染。该属已鉴定出11种，即M. globosa，M. restricta，M. sympodialis、合柄霉M. furfur，M. obtusa，M. slooffiae，M. pachyderplum，M. dermatal，M. japonica，M. yamatoensis、M.奶奶M.糠秕长期以来被认为是PV的致病真菌。然而，最近的研究利用培养和分离鉴定的形态和生理特性表明，M。球状体是PV的病原体。目的：本研究的目的是用基于分子的非培养方法检测PV微生物的分布。患者：受试者为49例PV患者（32例男性，17例女性; 16-83岁），他们在我们的门诊就诊。方法：采用亚甲基蓝直接镜检法，检测马拉色菌属，不检测马拉色菌。pachyderidae和M. nana使用非培养为基础的方法组成的巢式PCR与特异性引物。结果：最常见的分离种是M。globosa和M.局限性（均为93.9%）。只有M。在显微镜下仅观察到菌丝体的病斑中检测到球状体，而M.限制不是。结论：我们的结果表明，M。球状体是PV的病原体。
Background: Pityriasis versicolor (PV) is a superficial infection of the stratum corneum caused by Malassezia species. Eleven species have been identified within this genus, namely M. globosa, M. restricta, M. sympodialis, M. furfur, M. obtusa, M. slooffiae, M. pachydermatis, M. dermatis, M. japonica, M. yamatoensis, M. nana. M. furfur has long been identified as the causative fungus of PV. However, recent studies using the culture and isolation identified by morphological and physiological characteristics suggest that M. globosa is the causative agent of PV. Objectives: The aim of this study was to examine the distribution of PV microorganisms with a molecular-based non-culture method. Patients: The subjects were 49 patients with PV (32 males, 17 females; 16-83 years old) who visited our outpatient clinic. Methods: Samples were taken from lesions for direct microscopy with methylene blue and detected Malassezia species without M. pachydermatis and M. nana using a non-culture-based method consisting of nested PCR with specific primers. Results: The most frequently isolated species were M. globosa and M. restricta (both 93.9%). Only M. globosa was detected from the lesion in which the mycelial form alone was observed microscopically, but M. restricta was not. Conclusions: Our results suggest that M. globosa is the causative agent of PV.