HERPES-SIMPLEX VIRUSES LACKING GLYCOPROTEIN-D ARE UNABLE TO INHIBIT VIRUS PENETRATION - QUANTITATIVE EVIDENCE FOR VIRUS-SPECIFIC CELL-SURFACE RECEPTORS
HERPES-SIMPLEX VIRUSES LACKING GLYCOPROTEIN-D ARE UNABLE TO INHIBIT VIRUS PENETRATION - QUANTITATIVE EVIDENCE FOR VIRUS-SPECIFIC CELL-SURFACE RECEPTORS
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DOI：
10.1128/jvi.62.12.4605-4612.1988
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发表时间：
1988-12-01
影响因子：
5.4
通讯作者：
LIGAS, MW
中科院分区：
文献类型：
--
作者：
JOHNSON, DC;LIGAS, MW
单纯疱疹病毒（HSV）糖蛋白D （gD）在病毒进入细胞中起重要作用。构建了不能表达gD的HSV突变体。突变体可以在VD60细胞上繁殖，为病毒提供gD；然而，在突变体感染的Vero细胞中产生了缺乏gD的病毒颗粒。含有或不含gD的病毒颗粒被大量吸附（>.4倍）。104)个细胞表面的位点；然而，缺乏gD的病毒粒子没有进入细胞。用含有gD （.apprxeq）的uv灭活病毒粒子预处理的细胞。5个同学。103粒/细胞)对HSV 1型（HSV-1）和HSV-2感染具有抗性。相比之下，用缺乏gD的uv灭活病毒粒子预处理的细胞可以感染HSV-1和HSV-2。如果在含有gD的紫外线灭活病毒颗粒之前加入传染性HSV-1，传染性病毒进入细胞并复制。因此，含有gD的病毒颗粒似乎可以阻断数量非常有限的特定细胞表面受体。缺乏gD的颗粒可能无法与这些受体相互作用，这表明gD是一种必需的受体结合多肽。
Herpes simplex virus (HSV) glycoprotein D (gD) plays an essential role in the entry of virus into cells. HSV mutants unable to express gD were constructed. The mutants can be propagated on VD60 cells, which supply the viruses with gD; however, virus particles lacking gD were produced in mutant-infected Vero cells. Virus particles with or without gD adsorbed to a large number (> 4 .times. 104) of sites on the cell surface; however, virions lacking gD did not enter cells. Cells pretreated with UV-inactivated virions containing gD (.apprxeq. 5 .times. 103 particles per cell) were resistant to infection with HSV type 1 (HSV-1) and HSV-2. In contrast, cells pretreated with UV-inactivated virions lacking gD could be infected with HSV-1 and HSV-2. If infectious HSV-1 was added prior to UV-inactivated virus particles containing gD, the infectious virus entered cells and replicated. Therefore, virus particles containing gD appear to block specific cell surface receptors which are very limited in number. Particles lacking gD are presumably unable to interact with these receptors, suggesting that gD is an essential receptor-binding polypeptide.