Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection
Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection
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DOI：
10.1007/s00705-003-0188-4
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发表时间：
2003-12-01
影响因子：
2.7
通讯作者：
Domínguez, J
中科院分区：
文献类型：
--
作者：
Sánchez-Torres, C;Gómez-Puertas, P;Domínguez, J
非洲猪瘟病毒(ASFV)的靶细胞--单核巨噬细胞在表型和功能上具有高度的异质性。在这项研究中，我们调查了特定群体的猪巨噬细胞表型与其对ASFV感染的抵抗力之间的相关性。与肺泡巨噬细胞等成熟细胞相比，骨髓细胞和新鲜血液单核细胞在体外对ASFV感染的敏感性较低。用一组针对猪单核/巨噬细胞的单抗对单核细胞进行流式细胞仪分析表明，感染细胞具有更成熟的表型，表达更高水平的几种巨噬细胞特异性标志和SLA II抗原。单核细胞成熟后感染细胞百分率增加，与CD163表达增强相关。分离CD163(+)和CD163(-)单核细胞表明CD163(+)亚群对ASFV感染的特异性敏感性。体内实验也表明CD163的表达与病毒感染密切相关。最后，mAb2A10和mAb4E9以剂量依赖的方式抑制ASFV感染和病毒颗粒与肺泡巨噬细胞的结合。综上所述，这些结果有力地表明CD163在ASFV感染猪单核/巨噬细胞的过程中发挥了作用。
Monocytes-macrophages, the target cells of African swine fever virus (ASFV) are highly heterogeneous in phenotype and function. In this study, we have investigated the correlation between the phenotype of specific populations of porcine macrophages and their permissiveness to ASFV infection. Bone marrow cells and fresh blood monocytes were less susceptible to in vitro infection by ASFV than more mature cells, such as alveolar macrophages. FACS analyses of monocytes using a panel of mAbs specific for porcine monocyte/macrophages showed that infected cells had a more mature phenotype, expressing higher levels of several macrophage specific markers and SLA II antigens. Maturation of monocytes led to an increase in the percentage of infected cells, which correlated with an enhanced expression of CD163. Separation of CD163(+) and CD163(-) monocytes demonstrated the specific sensitivity of the CD163(+) subset to ASFV infection. In vivo experiments also showed a close correlation between CD163 expression and virus infection. Finally, mAb 2A10 and, in a lower extent, mAb 4E9 were able to inhibit, in a dose-dependent manner, both ASFV infection and viral particle binding to alveolar macrophages. Altogether, these results strongly suggest a role of CD163 in the process of infection of porcine monocytes/macrophages by ASFV.