Magnetic nanoparticles of Fe3O4 enhance docetaxel-induced prostate cancer cell death.
Magnetic nanoparticles of Fe3O4 enhance docetaxel-induced prostate cancer cell death.
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DOI：
10.2147/ijn.s40766
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发表时间：
2013
影响因子：
8
通讯作者：
Watanabe M
中科院分区：
文献类型：
--
作者：
Sato A;Itcho N;Ishiguro H;Okamoto D;Kobayashi N;Kawai K;Kasai H;Kurioka D;Uemura H;Kubota Y;Watanabe M
多西紫杉醇（Docetaxel， DTX）是最重要的抗癌药物之一；然而，其副作用的严重程度削弱了其在临床的实际应用。磁性纳米Fe3O4 （MgNPs-Fe3O4）可以增强抗癌药物的递送和疗效。我们研究了MgNPs-Fe3O4或DTX单独或联合体外前列腺癌细胞生长的作用，以及细胞毒性作用的机制。MgNPs-Fe3O4引起DU145、PC-3和LNCaP细胞中活性氧水平的剂量依赖性增加；8-羟基脱氧鸟苷水平也升高。单独使用MgNPs-Fe3O4可降低LNCaP和PC-3细胞的活力；然而，MgNPs-Fe3O4增强了低剂量DTX对所有三种细胞系的细胞毒性作用。MgNPs-Fe3O4也增加了低剂量DTX处理后DU145细胞凋亡的百分比。MgNPs-Fe3O4和DTX均不影响DU145的核转录因子κB的表达；而联合处理可抑制核转录因子κB的表达。这些发现提供了mgnps - fe3o4 -低剂量DTX联合治疗前列腺癌的可能性，其不良反应有限。
Docetaxel (DTX) is one of the most important anticancer drugs; however, the severity of its adverse effects detracts from its practical use in the clinic. Magnetic nanoparticles of Fe3O4 (MgNPs-Fe3O4) can enhance the delivery and efficacy of anticancer drugs. We investigated the effects of MgNPs-Fe3O4 or DTX alone, and in combination with prostate cancer cell growth in vitro, as well as with the mechanism underlying the cytotoxic effects. MgNPs-Fe3O4 caused dose-dependent increases in reactive oxygen species levels in DU145, PC-3, and LNCaP cells; 8-hydroxydeoxyguanosine levels were also elevated. MgNPs-Fe3O4 alone reduced the viability of LNCaP and PC-3 cells; however, MgNPs-Fe3O4 enhanced the cytotoxic effect of a low dose of DTX in all three cell lines. MgNPs-Fe3O4 also augmented the percentage of DU145 cells undergoing apoptosis following treatment with low dose DTX. Expression of nuclear transcription factor κB in DU145 was not affected by MgNPs-Fe3O4 or DTX alone; however, combined treatment suppressed nuclear transcription factor κB expression. These findings offer the possibility that MgNPs-Fe3O4–low dose DTX combination therapy may be effective in treating prostate cancer with limited adverse effects.