Comparison of six methods of analysis for the determination of aflatoxin B1 in feeding stuffs containing citrus pulp.
Comparison of six methods of analysis for the determination of aflatoxin B1 in feeding stuffs containing citrus pulp.
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DOI：
10.1080/02652038809373711
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发表时间：
1988-07
期刊：
影响因子：
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通讯作者：
H. V. van Egmond;W. E. Paulsch;E. Sizoo
中科院分区：
文献类型：
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作者：
H. V. van Egmond;W. E. Paulsch;E. Sizoo
比较了六种已发表的测定黄曲霉毒素B1的分析方法，以确定它们是否适合测定含柑橘果肉的饲料中的黄曲霉毒素B1。这些方法是欧洲共同体（EC）的官方程序，欧洲共同体提出的四种程序，以取代这种方法和本文作者开发的新程序。在所有程序中，氯仿用于初始提取。然后应用各种清洁系统。对于最终的分离和检测，在三个程序中使用高效液相色谱法（HPLC），在两个程序中使用二维薄层色谱法（TLC）。一种方法允许HPLC或TLC。所有的实验都是用一批含有柑橘果肉的饲料样品进行的，所述饲料样品分别被黄曲霉毒素B1、B2、G1和G2以约13、5、10和4微克/千克的水平人工污染。三种方法被认为是合适的：一个程序，其中使用凝胶渗透清洁和二维TLC;一个程序，其中使用TLC清洁和反相HPLC与柱后衍生化：一个程序，其中使用墨盒清洁和HPLC或TLC。后一种方法是优选的，因为其有效的净化产生非常干净的提取物，允许应用各种HPLC或TLC系统。在约10微克/千克饲料水平下，这三种方法对黄曲霉毒素B1的已公布回收率数据为85-90%。
Six published methods of analysis for the determination of aflatoxin B1 have been compared for their suitability to determine aflatoxin B1 in feeding stuffs containing citrus pulp. These methods are the official European Community (EC) procedure, four procedures proposed in the European Community to replace this method and a new procedure developed by the authors of this article. In all procedures chloroform is used for initial extraction. Various clean-up systems are then applied. For the ultimate separation and detection, use is made of high performance liquid chromatography (HPLC) in three procedures and two-dimensional thin layer chromatography (TLC) in two procedures. One method allows either HPLC or TLC. All experiments were carried out with samples of a batch of feeding stuff containing citrus pulp, artificially contaminated with aflatoxins B1, B2, G1 and G2 at levels of ca 13, 5, 10 and 4 micrograms/kg respectively. Three methods were found to be suitable: a procedure in which gel permeation clean-up and two-dimensional TLC are used; a procedure in which TLC clean-up and reverse phase HPLC with postcolumn derivatization are used: a procedure in which cartridge clean-up and either HPLC or TLC are used. The latter method is preferred because its efficient clean-up yields a very clean extract, allowing the application of various systems of HPLC or TLC. Published recovery data of these three methods for aflatoxin B1 vary from 85-90% at a level of ca 10 micrograms/kg feeding stuff.