Nicotinamide methyltransferase inhibition by S-adenosylethionine.
Nicotinamide methyltransferase inhibition by S-adenosylethionine.
复制标题
S-腺苷硫氨酸抑制烟酰胺甲基转移酶。
DOI：
10.1016/0304-3835(83)90167-2
复制
发表时间：
1983
期刊：
影响因子：
9.7
通讯作者：
Cox,R
中科院分区：
文献类型：
--
作者：
Cox,R
本文采用Affi-Gel 601柱分离烟酰胺甲基转移酶的方法，研究了S-腺苷硫代磷酸和S-腺苷高半胱氨酸对烟酰胺甲基化酶的抑制作用。5倍以上的S-腺苷硫代半胱氨酸比S-腺苷同型半胱氨酸是必要的50%的抑制酶。来自底物浓度曲线的数据支持两种抑制剂的竞争性抑制类型。有人提出，这种抑制可能会改变肝脏水平的烟酰胺和尿排泄的N1-甲基烟酰胺。
An assay for nicotinamide methyltransferase which uses an Affi-Gel 601 boronate column to separate substrate from product has been used to study the inhibition of the nicotinamide methylase.S-Adenosylethionine andS-adenosylhomocysteine inhibited the enzyme in a dose dependent fashion. Five times moreS-adenosylethionine thanS-adenosylhomocysteine was necessary for 50% inhibition of the enzyme. Data from a substrate concentration curve supports a competitive type of inhibition for both inhibitors. It is proposed that this inhibition may alter liver levels of nicotinamide and the urinary excretion ofN1-methylnicotinamide.