Transforming growth factor-β1 induces LMO7 while enhancing the invasiveness of rat ascites hepatoma cells
Transforming growth factor-β1 induces LMO7 while enhancing the invasiveness of rat ascites hepatoma cells
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DOI：
10.1016/j.canlet.2004.07.023
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发表时间：
2005-03-18
期刊：
影响因子：
9.7
通讯作者：
Miyoshi, J
中科院分区：
文献类型：
--
作者：
Nakamura, H;Mukai, M;Miyoshi, J
我们以前已经表明，转化生长因子-β 1（TGF-β 1）显着刺激大鼠腹水肝癌AH 130 W1细胞在体外和体内的侵袭能力。使用差异杂交程序分离在TGF-β 1处理的W1细胞中特异性上调的基因。在10个独立的cDNA克隆中，我们专注于LMO 7和通过选择性剪接产生的变体同种型LMO 7S。LMO 7具有PDZ和LIM结构域，而LMO 7S仅具有PDZ结构域。TGF-β 1上调LMO 7和LMO 7S的表达水平。LMO 7表达在高转移性克隆MM 1中上调。(c)2004爱思唯尔爱尔兰有限公司保留所有权利。
We have previously shown that transforming growth factor-beta 1 (TGF-beta 1) markedly stimulates the invasive capacity of rat ascites hepatoma AH130 W1 cells in vitro and in vivo. A differential hybridization procedure was used to isolate genes that were specifically up-regulated in TGF-beta 1 treated W1 cells. Among ten independent cDNA clones, we focused on LMO7 and a variant isoform, LMO7S, that was generated by alternative splicing. LMO7 had PDZ and LIM domains, while LMO7S had only PDZ domain. TGF-beta 1 up-regulated expression levels of LMO7 and LMO7S. LMO7 expression was up-regulated in the highly metastatic clone MM1. (c) 2004 Elsevier Ireland Ltd. All rights reserved.