An odorant receptor mediates the attractiveness of cis‐jasmone to Campoletis chlorideae, the endoparasitoid of Helicoverpa armigera
An odorant receptor mediates the attractiveness of cis‐jasmone to Campoletis chlorideae, the endoparasitoid of Helicoverpa armigera
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DOI：
10.1111/imb.12523
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发表时间：
2018-10
影响因子：
2.6
通讯作者：
Y.‐L. Sun;J.‐F. Dong;C. Ning;P-P Ding-P;L-Q. Huang;J. Sun;C.‐Z. Wang
中科院分区：
文献类型：
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作者：
Y.‐L. Sun;J.‐F. Dong;C. Ning;P-P Ding-P;L-Q. Huang;J. Sun;C.‐Z. Wang
寄生蜂依靠嗅觉在复杂的化学环境中找到它们的宿主。气味受体（ORs）与保守的气味辅助受体（ORcos）一起起作用，以确定嗅觉接收的灵敏度和特异性。棉铃虫凹腹姬蜂Campoletis chlorideae（Hyplutera：Ichneunmonidae）是棉铃虫Helicoverpa armigera等棉铃虫的一种单生幼虫内寄生蜂。了解C. chlorideae的嗅觉接收，我们测序成年男性和女性头部（包括触角）的转录组，并确定了211个OR转录本，其中95个是puppus全长。通过逆转录PCR评估的组织表达谱显示，7个OR仅在雌性触角中表达或表达更高。使用Xenopu slaevis卵母细胞表达系统和双电极电压钳记录分析其功能。CchlOR 62被调节为顺式茉莉酮，其对雌性C. chlorideae成虫和H.棉铃虫幼虫在随后的行为测定。进一步的生物测定表明，该菌在田间的寄生率为100%，在田间的寄生率为100%。棉铃虫C.在顺式茉莉酮处理的烟草植物上的chlorideae高于对照植物。因此，顺式茉莉酮似乎是一个重要的信息化学物质参与植物，H。armigera和C. chlorideae，而CchlOR 62介导了顺式茉莉酮对C. chlorideae。
Parasitic wasps rely on olfaction to locate their hosts in complex chemical environments. Odorant receptors (ORs) function together with well‐conserved odorant coreceptors (ORcos) to determine the sensitivity and specificity of olfactory reception. Campoletis chlorideae (Hymenoptera: Ichneunmonidae) is a solitary larval endoparasitoid of the cotton bollworm, Helicoverpa armigera, and some other noctuid species. To understand the molecular basis of C. chlorideae’s olfactory reception, we sequenced the transcriptome of adult male and female heads (including antennae) and identified 211 OR transcripts, with 95 being putatively full length. The tissue expression profiles, as assessed by reverse‐transcription PCR, showed that seven ORs were expressed only or more highly in female antennae. Their functions were analysed using the Xenopu slaevis oocyte expression system and two‐electrode voltage‐clamp recordings. CchlOR62 was tuned to cis‐jasmone, which was attractive to female C. chlorideae adults and H. armigera larvae in the subsequent behavioural assays. Further bioassays using caged plants showed that the parasitism rate of H. armigera larvae by C. chlorideae on cis‐jasmone‐treated tobacco plants was higher than on the control plants. Thus, cis‐jasmone appears to be an important infochemical involved in the interactions of plants, H. armigera and C. chlorideae, and CchlOR62 mediates the attractiveness of cis‐jasmone to C. chlorideae.