Fluid percussion injury transiently increases then decreases brain oxygen consumption in the rat.
Fluid percussion injury transiently increases then decreases brain oxygen consumption in the rat.
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DOI：
10.1089/neu.2000.17.101
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发表时间：
2000
影响因子：
4.2
通讯作者：
J. Levasseur;B. Alessandri;M. Reinert;R. Bullock;H. Kontos
中科院分区：
文献类型：
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作者：
J. Levasseur;B. Alessandri;M. Reinert;R. Bullock;H. Kontos
用笛卡尔微量呼吸计测定假手术组和侧位液压冲击伤（FPI）后30 min和6 h脑损伤组的氧耗量（VO 2 μ L/h/mg）。在损伤平面切割脑切片，并将特定部位的20-60 μ g组织核心转移到微量呼吸计中。在假手术组中，皮质VO 2（CVO 2）为13.78+/-0.64，海马VO 2（HPVO 2）为11.20+/-0.58 microL/h/mg（p<0.05）。在损伤后30分钟内，分别为16.89+/-0.55和14.91+/-0.06的值显著增加（p<0.05）。假手术组的合并VO 2（CVO 2，HPVO 2）为12.49+/-0.06 microL/h/mg，显著低于FPI后30分钟的合并VO 2 15.90+/-0.59 microL/h/mg（p<0.001）。最大CVO 2为19.49+/-1.10 microL/h/mg，最大HPVO 2为15.98+/-0.99 microL/h/mg，均来自损伤同侧。而对侧皮质损伤的大脑的值与假的没有显着差异，同侧和对侧海马的值显着大于假的响应于损伤（p<0.05）。到伤后6小时，合并的VO 2下降到10.01+/-0.84 microL/h/mg，但并不显著低于假手术值。数据表明，正常CVO 2大于正常HPVO 2。FPI使CVO 2和HPVO 2显著增加。此外，虽然CVO 2的立即增加似乎是损伤部位依赖性的，即区域性的，但HPVO 2的增加似乎是全球性的。
The oxygen consumption (VO2 microL/h/mg) of sham and of traumatized rat brains within 30 min and 6 h after a lateral fluid percussion injury (FPI) was measured with the Cartesian microrespirometer. Brain slices were cut at the plain of injury and site-specific 20-60-microg cores of tissue were transferred to the microrespirometer. In sham brains, the cortical VO2 (CVO2) was 13.78+/-0.64 and the hippocampal VO2 (HPVO2) was 11.20+/-0.58 microL/h/mg (p<0.05). Within 30 min of the injury, the respective values of 16.89+/-0.55 and 14.91+/-0.06 were significantly increased (p<0.05). The combined VO2 (CVO2, HPVO2) of 12.49+/-0.06 microL/h/mg in shams was significantly less than the combined VO2 of 15.90+/-0.59 microL/h/mg at 30 min post FPI (p<0.001). The maximal CVO2 of 19.49+/-1.10 microL/h/mg and the maximal HPVO2 of 15.98+/-0.99 microL/h/mg were both obtained from the ipsilateral side of the injury. Whereas the contralateral cortical value for injured brains was not significantly different from that of the shams, both ipsilateral and contralateral hippocampal values were significantly greater than that of the shams in response to injury (p<0.05). By 6 h postinjury, the combined VO2 had dropped to 10.01+/-0.84 microL/h/mg but was not significantly lower than the sham values. The data indicate that normal CVO2 is greater than normal HPVO2. The FPI produces significant increases in both CVO2 and HPVO2. Also, while the immediate increase in CVO2 appears to be injury-site dependent, that is, regional, the increase in HPVO2 appears to be global.