Gene therapy strategies to improve strength and quality of flexor tendon healing
Gene therapy strategies to improve strength and quality of flexor tendon healing
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提高屈肌腱愈合强度和质量的基因治疗策略
DOI：
10.1517/14712598.2016.1134479
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发表时间：
2016
影响因子：
4.6
通讯作者：
Wang Xiao Tian
中科院分区：
文献类型：
--
作者：
Tang Jin Bo;Zhou You Lang;Wu Ya Fang;Liu Paul Y.;Wang Xiao Tian
摘要简介：肌腱修复断裂和肌腱周围粘连是肌腱手术后的两个主要问题。促进愈合和减少粘连的新型生物疗法是许多研究的目标。基因治疗为解决这些难题提供了一种新的有前途的方法。在过去的十年中，我们试图通过基因治疗来开发增强肌腱愈合和减少肌腱粘连的方法。涵盖的领域：这篇综述讨论了腺相关病毒（AAV）2型载体基因治疗的方法和结果，以增加肌腱愈合强度和减少粘连的鸡模型。本文还讨论了micro-RNA相关的基因治疗。我们还开发了一种控释系统，该系统结合了纳米颗粒来递送微RNA以调节肌腱愈合。专家意见：我们在使用AAV 2介导的基因转移的鸡模型中获得了增强肌腱愈合强度的有希望的结果。AAV 2介导的micro-RNA转移也限制了肌腱周围的粘连。结合纳米颗粒的控释系统理想地将基因递送到愈合肌腱，并导致适度（但不完全）减少粘连。最佳剂量是什么以及在粘连形成中起作用的其他因素仍有待确定。这是我们今后调查的两个目标。
ABSTRACT Introduction: Rupture of the repair and adhesion around a tendon are two major problems after tendon surgery. Novel biological therapies which enhance healing and reduce adhesions are goals of many investigations. Gene therapy offers a new and promising approach to tackle these difficult problems. In the past decade, we sought to develop methods to augment tendon healing and reduce tendon adhesion through gene therapy. Areas covered: This review discusses the methods and results of adeno-associated viral (AAV) type 2 vector gene therapy to increase tendon healing strength and reduce adhesions in a chicken model. Micro-RNA related gene therapy is also discussed. We also developed a controlled release system, which incorporates nanoparticles to deliver micro-RNAs to regulate tendon healing. Expert opinion: We obtained promising results of enhancement of tendon healing strength in a chicken model using AAV2-mediated gene transfer. AAV2-mediated micro-RNA transfer also limited adhesions around the tendon. Controlled release systems incorporating nanoparticles have ideally delivered genes to the healing tendons and resulted in a moderate (but incomplete) reduction of adhesions. It remains to be determined what the best doses are and what other factors are in play in adhesion formation. These are two targets in our future investigations.