Long-term stability of conformation and redox response of cross-linked cytochrome c in polymer solvent
Long-term stability of conformation and redox response of cross-linked cytochrome c in polymer solvent
复制标题
交联细胞色素c在聚合物溶剂中的构象和氧化还原反应的长期稳定性
DOI：
10.1016/s0013-4686(00)00367-4
复制
发表时间：
2000
影响因子：
6.6
通讯作者：
H. Ohno
中科院分区：
文献类型：
--
作者：
F. Kurusu;H. Ohno
聚环氧乙烷(PEO)修饰的细胞色素c-戊二醛(PEO-GA-cyt.c)在PEO齐聚物中保持氧化还原活性约1年。PEO-GA-cyt.c通过电化学吸附固定在ITO玻璃电极上的峰电流逐渐减小，半年后没有观察到氧化还原反应。PEO-GA-cyt.c在PEO-200(平均分子质量为200，含0.5mol/L KCl)中的CD谱表明，在大约1年的时间里没有发生大的构象变化。PEO-GA-cyt.c稳定性的提高归功于与GA的交联性和PEO作为溶剂的使用。
Poly(ethylene oxide) (PEO) modified cytochrome c cross-linked with glutaraldehyde (PEO-GA-cyt.c) kept redox activity in PEO oligomers for about 1 year. The peak current for PEO-GA-cyt.c fixed on the ITO glass electrode by the electrochemical adsorption gradually decreased and no redox response was observed about a half year later. A CD spectrum for PEO-GA-cyt.c in PEO200(average molecular weight of 200, containing 0.5 mol/l KCl) suggested that a large conformational change did not occur for about 1 year. This improved stability of PEO-GA-cyt.c was considered attributed to both the cross-linking with GA and the use of PEO as a solvent.