SET DOMAIN GROUP 708, a histone H3 lysine 36-specific methyltransferase, controls flowering time in rice (Oryza sativa)
SET DOMAIN GROUP 708, a histone H3 lysine 36-specific methyltransferase, controls flowering time in rice (Oryza sativa)
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SET DOMAIN GROUP 708 是一种组蛋白 H3 赖氨酸 36 特异性甲基转移酶，控制水稻 (Oryza sativa) 的开花时间
DOI：
10.1111/nph.13768
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发表时间：
2016-04-01
期刊：
影响因子：
9.4
通讯作者：
Dong, Aiwu
中科院分区：
文献类型：
--
作者：
Liu, Bing;Wei, Gang;Dong, Aiwu
组蛋白H3赖氨酸36（H3 K36）甲基化作为一种重要的表观遗传修饰，调节染色质结构并参与多种生物学过程。为了更好地理解水稻中H3 K36甲基化的语言，我们选择了潜在的组蛋白甲基化酶进行功能探索。特别地，我们将水稻SET结构域组708（SDG 708）表征为具有在H3 K36上存款多达三个甲基的能力的H3 K36特异性甲基转移酶。与野生型相比，SDG 708基因敲除的水稻突变体在长日照和短日照条件下均表现出晚花表型，这是由于其关键开花调控基因抽穗期3a（Hd 3a）、水稻开花基因座T1（RFT 1）和早抽穗期1（Ehd 1）的下调所致。染色质免疫沉淀实验表明，H3 K36 me 1，H3 K36 me 2和H3 K36 me 3水平在SDG 708缺陷型植物中的这些位点降低。更重要的是，SDG 708能够直接靶向并影响特定开花基因上的H3 K36甲基化。事实上，SDG 708的敲低导致了一组功能基因的错误表达，以及水稻生长早期H3 K36 me 1/2/3水平的全基因组下降。SDG 708是一种甲基转移酶，催化H3 K36上所有三个甲基的全基因组沉积，并且除了促进开花之外还参与许多生物学过程。
As a key epigenetic modification, the methylation of histone H3 lysine 36 (H3K36) modulates chromatin structure and is involved in diverse biological processes. To better understand the language of H3K36 methylation in rice (Oryza sativa), we chose potential histone methylation enzymes for functional exploration. In particular, we characterized rice SET DOMAIN GROUP 708 (SDG708) as an H3K36-specific methyltransferase possessing the ability to deposit up to three methyl groups on H3K36. Compared with the wild-type, SDG708-knockdown rice mutants displayed a late-flowering phenotype under both long-day and short-day conditions because of the down-regulation of the key flowering regulatory genes Heading date 3a (Hd3a), RICE FLOWERING LOCUS T1 (RFT1), and Early heading date 1 (Ehd1). Chromatin immunoprecipitation experiments indicated that H3K36me1, H3K36me2, and H3K36me3 levels were reduced at these loci in SDG708-deficient plants. More importantly, SDG708 was able to directly target and effect H3K36 methylation on specific flowering genes. In fact, knockdown of SDG708 led to misexpression of a set of functional genes and a genome-wide decrease in H3K36me1/2/3 levels during the early growth stages of rice. SDG708 is a methyltransferase that catalyses genome-wide deposition of all three methyl groups on H3K36 and is involved in many biological processes in addition to flowering promotion.