MAPPING OF THE REGION PREDOMINANTLY RECOGNIZED BY ANTIBODIES TO THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE-ANTIGEN MSA-1
MAPPING OF THE REGION PREDOMINANTLY RECOGNIZED BY ANTIBODIES TO THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE-ANTIGEN MSA-1
复制标题
DOI：
10.1016/0166-6851(92)90080-4
复制
发表时间：
1992-04-01
影响因子：
1.5
通讯作者：
SAUL, AJ
中科院分区：
文献类型：
--
作者：
COOPER, JA;COOPER, LT;SAUL, AJ
通过使用天然存在的加工片段、通过蛋白质的化学切割以及通过比较结合的分离株特异性与已知序列的比较，绘制了一组针对恶性疟原虫裂殖子表面抗原MSA 1的小鼠单克隆抗体的表位位置。变异。根据这些标准，最具抗原性的区域出现在富含半胱氨酸的不变的19 kDa羧基末端结构域中，12/19的单克隆抗体（mAb）与该区域结合。这些单克隆抗体之一识别C-末端推定的糖基磷脂酰肌醇锚位点附近的表位。这是在体外显著抑制寄生虫生长的唯一mAb。其他单克隆抗体识别构象表位，涉及位于整个该片段的半胱氨酸残基。这项研究已经确定了进一步的自然发生的加工位点和共识加工位点序列现在正在出现。
The locations of the epitopes of a panel of mouse monoclonal antibodies directed against the Plasmodium falciparum merozoite surface antigen MSA 1 were mapped by using naturally occurring processed fragments, by chemical cleavage of the protein and by comparison of the isolate-specificity of binding with known sequence variation. By these criteria, the most antigenic region occurs in the cysteine-rich, invariant 19-kDa carboxyl terminal domain with 12/19 monoclonal antibodies (mAbs) binding to this region. One of these mAbs recognized an epitope near the C-terminal putative glycosylphosphatidylinositol anchor site. This was the only mAb which significantly inhibited parasite growth in vitro. The other mAbs recognized conformational epitopes involving the cysteine residues located throughout this fragment. This study has identified further naturally occurring processing sites and a consensus processing site sequence is now emerging.