Evaluation of Metronidazole With and Without Enterococcus Faecium SF68 in Shelter Dogs With Diarrhea
Evaluation of Metronidazole With and Without Enterococcus Faecium SF68 in Shelter Dogs With Diarrhea
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DOI：
10.1053/j.tcam.2017.11.001
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发表时间：
2017-09-01
影响因子：
1.1
通讯作者：
Lappin, Michael R.
中科院分区：
文献类型：
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作者：
Fenimore, Audra;Martin, Laura;Lappin, Michael R.
腹泻在收容所犬和非特异性治疗中很常见，如治疗性饮食，益生菌和抗贾第虫药物。或肠道细菌通常凭经验开处方。本研究中的所有狗均给予甲硝唑，喂食标准化饮食，并随机接受市售益生菌（Purina（R）Pro Plan（R）Veterinary Diets; FortiFlora（R）Probiotic Supplement，FortiFlora，Nestle Purina PetCare，圣刘易斯，MO）或安慰剂，安慰剂是不含益生菌的市售产品，持续7天。对设盲个体在研究期间排出的每份粪便进行粪便评分。评价粪便样品中选择的肠溶剂，包括线虫、贾第虫属，隐孢子虫属，和产气荚膜梭菌肠毒素。在寄生虫感染率方面，两组之间没有差异。到第7天，在37.5%的甲硝唑给药犬和68.8%的双重治疗犬中检测到正常粪便（<5），但结果不显著（P = 0.1556）。当与仅给予甲硝唑的犬（46.9%）相比时，给予双重治疗的犬的正常粪便天数百分比显著更高（P = 0.0496）（65.6%）。在SF68组中感染的两只犬中，贾第虫包囊被消除，腹泻消退。相比之下，在安慰剂组的7只贾第虫阳性犬中，6只（85.7%）在第7天仍为贾第虫包囊阳性，其中4只犬在第7天仍有腹泻。添加SF68的甲硝唑和标准化的饮食的协议，似乎提高了收容所狗腹泻的临床反应。(C)2017爱思唯尔公司All rights reserved.
Diarrhea is common in shelter dogs and nonspecific therapies like therapeutic diets, probiotics, and drugs with activity against Giardia spp. or enteric bacteria are commonly prescribed empirically. All dogs in this study were administered metronidazole, fed a standardized diet, and randomized to either receive a commercially available probiotic (Purina (R) Pro Plan (R) Veterinary Diets; FortiFlora (R) Probiotic Supplement, FortiFlora, Nestle Purina PetCare, St Louis, MO) or a placebo which was the commercial product without the probiotic for 7 days. A fecal score was assigned to each stool passed during the study by masked individuals. Fecal samples were evaluated for select enteric agents including nematodes, Giardia spp., Cryptosporidium spp., and Clostridium perfringens enterotoxin before and at the end of the treatment period. There were no differences between groups in regard to parasite prevalence. By day 7, a normal stool (< 5) was detected in 37.5% of the dogs administered metronidazole and 68.8% of the dogs administered dual therapy, but the result was not significant (P = .1556). The percentages of days with normal stools were significantly higher (P = .0496) for dogs administered dual therapy 65.6%) when compared to those administered metronidazole alone (46.9%). Giardia cysts were eliminated and diarrhea resolved in both dogs that were infected in the SF68 group. In contrast, of the 7 Giardia positive dogs in the placebo group, 6 (85.7%) were still positive for Giardia cysts on day 7, and 4 of those dogs still had diarrhea on day 7. Addition of SF68 to this protocol of metronidazole and a standardized diet appeared to enhance clinical responses in shelter dogs with diarrhea. (C) 2017 Elsevier Inc. All rights reserved.