Characterization of atypical Aeromonas salmonicida by different methods
Characterization of atypical Aeromonas salmonicida by different methods
复制标题
DOI：
10.1016/s0723-2020(98)80008-8
复制
发表时间：
1998-03-01
影响因子：
3.4
通讯作者：
Powell, R
中科院分区：
文献类型：
--
作者：
Austin, B;Austin, DA;Powell, R
52株非典型杀鲑气单胞菌，回收范围广泛的主机和地理位置，是异质性的分子和表型特征，并代表类群不能容纳目前的分类四个亚种。一般来说，有不一致的分子（PCR，RAPD和核糖分型）和表型方法的聚类成员。通过PCR扩增，共得到6个群，其中群1包括12个分离株，包括A.杀鲑亚种，史密斯亚种第2组容纳了23株分离株，包括非产色菌亚种和杀螨亚种的参考培养物。在第6组中回收了指定的鱼嗜血杆菌培养物。通过核糖分型和RAPD，参考培养物被回收到不同的组中。大多数分离物含有2-6个质粒，长度为2.3 - 150 kb。然而，所有分离株都具有某些关键特征，包括革兰氏阴性和缺乏运动性。
Fifty two isolates of atypical Aeromonas salmonicida, recovered from a wide range of hosts and geographical locations, were heterogeneous in terms of molecular and phenotypic characteristics, and represented taxa which could not be accommodated by the current classification of four subspecies. Generally, there was incongruence between the molecular (PCR, RAPD and ribotyping) and phenotypic methods in terms of cluster membership. By PCR, 6 groups were described of which Group 1 encompassed 12 isolates including the type strain of A. salmonicida subsp, smithia. Group 2 accommodated 23 isolates including the reference cultures of subspecies achromogenes and masoucida. The named culture of Haemophilus piscium was recovered in Group 6. By ribotyping and RAPD, the reference cultures were recovered in separate groups. All methods pointed to the uniqueness of subspecies smithia, Most isolates contained 2-6 plasmids, of 2.3 to 150 kb in length. Nevertheless, ail isolates possessed certain key characteristics, including Gram-negativity, and the absence of motility.