Estrogen receptor alpha and beta expression in upper gastrointestinal tract with regulation of trefoil factor family 2 mRNA levels in ovariectomized rats
Estrogen receptor alpha and beta expression in upper gastrointestinal tract with regulation of trefoil factor family 2 mRNA levels in ovariectomized rats
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DOI：
10.1006/bbrc.1997.7683
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发表时间：
1997-11-17
影响因子：
3.1
通讯作者：
CampbellThompson, ML
中科院分区：
文献类型：
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作者：
CampbellThompson, ML
本研究比较了两种雌激素受体（ER α和ER β）基因在大鼠上消化道的表达和17 β-雌二醇给药对卵巢切除大鼠胃三叶因子家族（TFF）mRNA稳态水平的影响。采用逆转录聚合酶链反应（RT-PCR）技术从胃底粘膜中克隆雌激素受体α和β cDNA片段，并进行序列测定。RT-PCR法检测胃底、胃窦和十二指肠的ER亚型。北方分析表明，ER α mRNA以6.5kb的单一转录本表达，ER β以1.1- 4.7kb的多转录本表达。ER β mRNA的表达丰度高于ER α mRNA。在卵巢切除大鼠中，给予17 β-雌二醇可增加胃底TFF 2 mRNA稳态水平，而TFF 1 mRNA水平无显著变化。这些研究表明，两种ER亚型在大鼠上消化道中的表达与17 β-雌二醇对TFF 2 mRNA的调节有关。这些结果表明，雌激素，可能通过ER β发挥作用，在调节胃生理学有直接的作用。(C)北京：科学出版社.
This study compared expression of two estrogen receptor (ER alpha and ER beta) genes in the rat upper gastrointestinal tract and the effects of 17 beta-estradiol administration on gastric trefoil factor family (TFF) mRNA steady-state levels in ovariectomized rats. Estrogen receptor alpha and beta cDNA fragments from fundic mucosa were cloned by reverse transcriptase polymerase chain reaction (RT-PCR) and sequenced. Both ER subtypes were detected in fundus, antrum and duodenum by RT-PCR. Northern analysis of poly(A)(+) mRNA hom fundic mucosa showed that ER alpha mRNA is expressed as a single transcript at 6.5 kb and ER beta is expressed as multiple transcripts with major transcripts ranging from 1.1-4.7 kb. ER beta mRNA was expressed in greater abundance than ER alpha mRNA. Fundic TFF2 mRNA steady-state levels were increased by 17 beta-estradiol administration in ovariectomized rats with no significant change in TFF1 mRNA levels. These studies show expression of both ER subtypes in the rat upper gastrointestinal tract with regulation of TFF2 mRNA by 17 beta-estradiol. These results suggest that estrogens, probably acting via ER beta, have a direct role in regulating gastric physiology. (C) 1997 Academic Press.