Distribution of periodontopathic bacterial species in Japanese children with developmental disabilities.
Distribution of periodontopathic bacterial species in Japanese children with developmental disabilities.
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DOI：
10.1186/1472-6831-9-24
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发表时间：
2009-09-23
期刊：
影响因子：
2.9
通讯作者：
Ooshima, Takashi
中科院分区：
文献类型：
--
作者：
Naka, Shuhei;Yamana, Aki;Ooshima, Takashi
背景技术背景：分子生物学技术的最新发展使得能够快速检测临床样本中的牙周病细菌种类。累积的证据表明，特定细菌种类的检测能够识别牙周炎发病的高风险受试者。我们调查了10个选定的牙周病细菌菌种的分布在牙菌斑标本中获得的残疾儿童谁是参加daycare centres.METHODS：共187名儿童（136名男孩，51名女孩），年龄1 - 6岁，诊断为精神发育迟滞，脑瘫，自闭症等残疾，参加了这项研究。临床检查后，从上颌左第二乳磨牙颊侧采集龈下菌斑标本。从标本中提取细菌DNA，并进行PCR分析，以检测10个选定的牙周病物种，使用每个特定的引物。结果：痰生二氧化碳嗜纤维菌（Capnocytophagasputigena）检出率最高（28.3%），其次为伴放线菌聚集杆菌（Aggregatibacteractinomycetemcomitans）（20.9%）和直弯杆菌（Campylobacterrectus）（18.2%）。腐蚀艾肯氏菌，二氧化碳嗜纤维菌赭色，和变黑普雷沃菌检测到约10%的标本，而齿垢密螺旋体，Tannerella asthia，和中间普雷沃菌很少发现，牙龈卟啉单胞菌没有检测到任何科目。检出的物种总数与受试者的年龄呈正相关。10例受试者T. denticola和/或T.与其他受试者相比，其中细菌种类的总数显著更高。此外，具有C.直肌表现出显着更大的值牙周袋深度，牙龈指数，和物种总数。结论：我们发现，约四分之一的残疾人目前的主题谁拥有至少一个T。denticola，齿齿叶蜂T. Aphthia和C.直肌可能有患牙周炎的风险。对这些人应采取后续检查和预防措施。
BACKGROUND: Recent developments in molecular biological techniques have enabled rapid detection of periodontopathic bacterial species in clinical specimens. Accumulated evidence suggests that detection of specific bacterial species enables identification of subjects at high risk for the onset of periodontitis. We investigated the distribution of 10 selected periodontopathic bacterial species in dental plaque specimens obtained from children with disabilities who were attending daycare centers.METHODS: A total of 187 children (136 boys, 51 girls) aged 1-6 years old and diagnosed with such disabilities as mental retardation, cerebral palsy, and autism, participated in the study. Subgingival dental plaque specimens were collected from the buccal side of the maxillary left second primary molar after a clinical examination. Bacterial DNA was extracted from the specimens and PCR analyses were carried out to detect 10 selected periodontopathic species using specific primers for each. In addition, statistical analyses were performed to analyze the correlations among clinical parameters and the detected species.RESULTS: The most frequently detected species was Capnocytophaga sputigena (28.3%), followed by Aggregatibacter actinomycetemcomitans (20.9%) and Campylobacter rectus (18.2%). Eikenella corrodens, Capnocytophaga ochracea, and Prevotella nigrescence were detected in approximately 10% of the specimens, whereas Treponema denticola, Tannerella forsythia, and Prevotella intermedia were rarely found, and Porphyromonas gingivalis was not detected in any of the subjects. The total numbers of detected species were positively correlated with the age of the subjects. There were 10 subjects with positive reactions for T. denticola and/or T. forsythia, in whom the total number of bacterial species was significantly higher as compared to the other subjects. Furthermore, subjects possessing C. rectus showed significantly greater values for periodontal pocket depth, gingival index, and total number of species.CONCLUSION: We found that approximately one-fourth of the present subjects with disabilities who possessed at least one of T. denticola, T. forsythia, and C. rectus were at possible risk for periodontitis. Follow-up examinations as well as preventive approaches should be utilized for such individuals.