Accumulation of Phenylpropanoid and Sesquiterpenoid Volatiles in Male Rectal Pheromonal Glands of the Guava Fruit Fly, Bactrocera correcta
Accumulation of Phenylpropanoid and Sesquiterpenoid Volatiles in Male Rectal Pheromonal Glands of the Guava Fruit Fly, Bactrocera correcta
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DOI：
10.1007/s10886-010-9874-3
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发表时间：
2010-12-01
影响因子：
2.3
通讯作者：
Nishida, Ritsuo
中科院分区：
文献类型：
--
作者：
Tokushima, Isao;Orankanok, Watchreeporn;Nishida, Ritsuo
番石榴实蝇Bactrocera correcta广泛分布于泰国和其他周边的东南亚国家，并且与密切相关的同域物种东方实蝇B一样。dorsalis，侵染各种水果，包括番石榴，桃和芒果。B组均为雄性。correcta和B。dorsalis强烈吸引，并强制饲料，甲基丁香酚（ME）。桔小实蝇雄性取食ME后，其代谢产物苯丙素类化合物（E）-松柏醇和2-烯丙基-4，5-二甲氧基苯酚在直肠信息素腺体中被隔离。相反，B.喂食ME的correcta雄性在直肠腺中螯合两种不同的代谢物，（Z）-松柏醇（ZCF）和（Z）-3，4-二甲氧基肉桂醇（DMC）。检查B中ME代谢物的时间变化。correcta雄性直肠腺显示，在ME饲喂后24小时，ZCF和DMC的总量高达100 μ g/雄性。ZCF和DMC在野生B中检出率较高。在泰国不同地点捕获的correcta雄性。从B开始。correcta和B。dorsalis在泰国是同域物种，这两个不同的直肠苯丙烷类化合物亚群可以发挥作用，以避免物种之间的杂交。对泰国野生苍蝇的进一步调查显示，大部分雄性B。correcta在直肠腺中储存大量（超过250 μ g/腺体）的倍半萜烃，包括β-香芹烯、α-蛇麻烯和别芳香马登烯，以及ZCF和DMC，或者代替ZCF和DMC。人工饲养的雄性也螯合β-香紫苏烯和α-蛇麻烯，沿着ZCF和DMC，当倍半萜与ME一起人工供应时。田间试验表明，β-香紫苏烯和α-蛇麻烯的混合物（1：1）吸引雄性B。correcta，虽然在较小的数量比在陷阱诱饵与ME。的螯合倍半萜，除了不同的ME代谢产物中的信息素腺体在B。与B的情况相反。dorsalis男性，这表明在这些同域物种之间的种内和/或种间相互作用的潜在作用。
The guava fruit fly, Bactrocera correcta, is widely distributed in Thailand and other surrounding Southeast Asian countries, and, like the closely related sympatric species, the oriental fruit fly, B. dorsalis, infests various fruits, including guava, peach, and mango. Males of both B. correcta and B. dorsalis are strongly attracted to, and compulsively feed on, methyl eugenol (ME). Bactrocera dorsalis males fed on ME sequester its metabolite phenylpropanoids, (E)-coniferyl alcohol and 2-allyl-4,5-dimethoxyphenol, in the rectal pheromone gland. In contrast, B. correcta males fed on ME sequester two different metabolites, (Z)-coniferyl alcohol (ZCF) and (Z)-3,4-dimethoxycinnamyl alcohol (DMC), in the rectal gland. Examination of the temporal changes of ME metabolites in B. correcta male rectal glands revealed that the total of ZCF and DMC was as high as 100 mu g/male at 24 hr after ME feeding. ZCF and DMC were detected in a large proportion of wild B. correcta males captured at various sites in Thailand. Since B. correcta and B. dorsalis are sympatric species in Thailand, these two different subsets of rectal phenylpropanoids could play a role to avoid interbreeding between the species. Further survey of wild flies in Thailand revealed that a large proportion of males of B. correcta store large quantities (over 250 mu g/gland) of sesquiterpene hydrocarbons, including beta-caryophyllene, alpha-humulene, and alloaromadendrene in the rectal gland in addition to, or instead of, ZCF and DMC. Laboratory-reared males also sequestered beta-caryophyllene and alpha-humulene, along with ZCF and DMC, when the sesquiterpenes were artificially supplied together with ME. A field test demonstrated that a mixture (1:1) of beta-caryophyllene and alpha-humulene attracted male B. correcta, albeit in smaller numbers than in traps baited with ME. The sequestration of sesquiterpenes, in addition to the different ME metabolites in the pheromone gland in B. correcta males, contrasts with the situation in B. dorsalis males, suggesting a potential role in intra and/or inter-specific interactions between these sympatric species.