Continuing evolution of H9N2 influenza viruses in Southeastern China
Continuing evolution of H9N2 influenza viruses in Southeastern China
复制标题
DOI：
10.1128/jvi.78.16.8609-8614.2004
复制
发表时间：
2004-08-01
影响因子：
5.4
通讯作者：
Guan, Y
中科院分区：
文献类型：
--
作者：
Choi, YK;Ozaki, H;Guan, Y
H9N2流感病毒在欧亚大陆的家禽中流行，自1999年以来已在人和猪中造成短暂感染。为了研究H9N2病毒的人畜共患可能性，我们研究了2003年香港活禽市场病毒的演变。H9N2是2001年至2003年期间活禽市场上最流行的流感病毒亚型。血凝素（HA）抗原分析和系统发育分析表明，除1株外，其余19株均属于A/Duck/Hong Kong/Y280/97 （H9N2）谱系。除A/Guinea fowl/NT184/03 （H9N2）外，其HA与人类分离株A/Guangzhou/333/99 （H9N2）的HA最接近，属于A/Chicken/Beijing/1/94 like （H9N2）谱系。至少有六种不同的基因型被识别出来。大多数病毒具有源自A/Duck/Hong Kong/ y280 /97样病毒谱系的非结构（和HA）基因，但具有其他源自混合禽流感病毒的基因，包括与2001年分离的H5N1病毒相似的基因。发现的所有六种H9N2基因型的病毒都能够在鸡和小鼠中不适应地复制。受感染的鸡没有出现疾病迹象，但两种病毒基因型的代表对小鼠是致命的。三种基因型的病毒在猪的呼吸道中复制，猪至少在5天内释放病毒。这些结果显示香港H9N2病毒的遗传和生物多样性日益增加，并支持它们作为大流行性流感病原体的潜在作用。
H9N2 influenza viruses are panzootic in domestic poultry in Eurasia and since 1999 have caused transient infections in humans and pigs. To investigate the zoonotic potential of H9N2 viruses, we studied the evolution of the viruses in live-poultry markets in Hong Kong in 2003. H9N2 was the most prevalent influenza virus subtype in the live-poultry markets between 2001 and 2003. Antigenic and phylogenetic analysis of hemagglutinin (HA) showed that all of the 19 isolates found except one belonged to the lineage represented by A/Duck/Hong Kong/Y280/97 (H9N2). The exception was A/Guinea fowl/NT184/03 (H9N2), whose HA is most closely related to that of the human isolate A/Guangzhou/333/99 (H9N2), a virus belonging to the A/Chicken/Beijing/1/94-like (H9N2) lineage. At least six different genotypes were recognized. The majority of the viruses had nonstructural (and HA) genes derived from the A/Duck/Hong Kong/Y280/97-like virus lineage but had other genes of mixed avian virus origin, including genes similar to those of H5N1 viruses isolated in 2001. Viruses of all six genotypes of H9N2 found were able to replicate in chickens and mice without adaptation. The infected chickens showed no signs of disease, but representatives of two viral genotypes were lethal to mice. Three genotypes of virus replicated in the respiratory tracts of swine, which shed virus for at least 5 days. These results show an increasing genetic and biologic diversity of H9N2 viruses in Hong Kong and support their potential role as pandemic influenza agents.