Antifungal susceptibilities of Cryptococcus species complex isolates from AIDS and non-AIDS patients in Southeast China
Antifungal susceptibilities of Cryptococcus species complex isolates from AIDS and non-AIDS patients in Southeast China
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DOI：
10.1590/s1413-86702012000200012
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发表时间：
2012-04-01
影响因子：
3.4
通讯作者：
Weng, Lixing
中科院分区：
文献类型：
--
作者：
Li, Meng;Liao, Yong;Weng, Lixing
隐球菌属是免疫功能低下患者真菌病的常见原因。目的：测定临床隐球菌属的药敏性。61株临床隐球菌的特征。对52株C. neoformans和9 C. 1993年至2009年在上海收集的格特分离株。采用微量稀释法M27-A2和AT B FUNGUS 3试剂盒测定临床分离菌对阿替霉素B、氟康唑、伊曲康唑和氟胞嘧啶的敏感性。90%最小抑菌浓度（MIC 90）和敏感性范围如下：阿替霉素B为1（0.0625-1）μ g/mL，氟康唑为4（0.125-16）μ g/mL，伊曲康唑为0.25（0.0313-4）μ g/mL，氟胞嘧啶为4（0.125-8）μ g/mL。氟康唑、伊曲康唑和氟胞嘧啶对所有测试的临床隐球菌属具有优异的体外活性，并且我们还发现了对阿替霉素B的高耐受率（MIC范围为0.55-1 μ g/mL）。此外，C.从获得性免疫缺陷综合征（AIDS）患者分离的新生儿比从非AIDS患者分离的新生儿对氟康唑和氟胞嘧啶的敏感性低。这些数据表明，随着时间的推移，使用阿替霉素B可能导致病原体的耐受性或耐药性。这些临床分离株的种属、基因型和体外敏感性之间也没有显著相关性。(C)2012 Elsevier Editora Ltda. All rights reserved.
Cryptococcus spp. are common causes of mycoses in immunocompromised patients. To determine the drug susceptibilities of clinical Cryptococcus spp. isolates, the characteristics of 61 clinical Cryptococcus spp. complex isolates and their antifungal susceptibilities were investigated, including 52 C. neoformans and 9 C. gattii isolates collected at Shanghai between 1993 and 2009. Antifungal susceptibility of clinical isolates to amphotericin B, fluconazole, itraconazole, and flucytosine were determined by the microdilution method M27-A2 and the ATB FUNGUS 3 kit. The 90% minimum inhibitory concentration (MIC90) and susceptibility ranges were as follows: 1 (0.0625-1) mu g/mL for amphotericin B, 4 (0.125-16) mu g/mL for fluconazole, 0.25 (0.0313-4) mu g/mL for itraconazole, and 4 (0.125-8) mu g/mL for flucytosine. Fluconazole, itraconazole, and flucytosine have excellent in vitro activity against all tested clinical Cryptococcus spp., and we also found a high rate of tolerance to amphotericin B (MICs ranging from 0.55-1 mu g/mL). Furthermore, C. neoformans isolates from acquired immune deficiency syndrome (AIDS) patients were less susceptible to fluconazole and flucytosine than those from non-AIDS patients. These data suggest that use of amphotericin B may lead to tolerance or resistance of the pathogen over time. There were also no significant associations between species, genotypes, and in vitro susceptibilities of these clinical isolates. (C) 2012 Elsevier Editora Ltda. All rights reserved.