The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase, Wzz, from enterobacterial common antigen biosynthesis form a complex.
The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase, Wzz, from enterobacterial common antigen biosynthesis form a complex.
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肠杆菌共同抗原（ECA）是肠杆菌科中与细胞包膜相关的碳水化合物聚合物。ECA含有由Wzy膜蛋白聚合酶超家族成员Wzy E聚合的重复三糖。WzyE活性受膜蛋白多糖共聚合酶WzzE调节。Förster共振能量转移实验表明，来自黑腐果胶杆菌的WzyE和WzzE在体内形成复合物，免疫印迹和冷冻电子显微镜（cryo-EM）分析证实了约8个WzzE与1个WzyE的定义化学计量。低分辨率的cryo-EM重建的复杂，由抗体识别的C-末端的WzyE，揭示WzyE位于中央膜腔形成的八聚体排列的跨膜螺旋的WzzE。Wzy和Wzz的配对在其他细菌聚合物的聚合系统中发现，包括脂多糖O-抗原和荚膜多糖。这些数据为调节细菌中多糖链长度的保守机制提供了新的结构见解。
The enterobacterial common antigen (ECA) is a carbohydrate polymer that is associated with the cell envelope in the Enterobacteriaceae. ECA contains a repeating trisaccharide which is polymerized by WzyE, a member of the Wzy membrane protein polymerase superfamily. WzyE activity is regulated by a membrane protein polysaccharide co-polymerase, WzzE. Förster resonance energy transfer experiments demonstrate that WzyE and WzzE from Pectobacterium atrosepticum form a complex in vivo, and immunoblotting and cryo-electron microscopy (cryo-EM) analysis confirm a defined stoichiometry of approximately eight WzzE to one WzyE. Low-resolution cryo-EM reconstructions of the complex, aided by an antibody recognizing the C-terminus of WzyE, reveals WzyE sits in the central membrane lumen formed by the octameric arrangement of the transmembrane helices of WzzE. The pairing of Wzy and Wzz is found in polymerization systems for other bacterial polymers, including lipopolysaccharide O-antigens and capsular polysaccharides. The data provide new structural insight into a conserved mechanism for regulating polysaccharide chain length in bacteria.
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影响因子：
3.2
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