Cervical ripening and parturition in cows are driven by a cascade of pro-inflammatory cytokines.
Cervical ripening and parturition in cows are driven by a cascade of pro-inflammatory cytokines.
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奶牛的宫颈成熟和分娩是由一系列促炎细胞因子驱动的。
DOI：
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
V. Rutten
中科院分区：
文献类型：
--
作者：
E. Engelen;M. Groot;V. Breeveld;M. Everts;G. V. D. Weyden;M. Taverne;V. Rutten;V. Rutten
宫颈成熟和分娩的最后阶段类似于炎症过程。虽然细胞因子在自发和实验诱导分娩中的作用已经在一些小型实验动物和人类中得到描述，但促炎和调节细胞因子在奶牛生理分娩中的参与尚未确定。在这项研究中，评估了妊娠和分娩几个阶段的牛宫颈组织中的细胞因子表达谱。在怀孕第 185 天和第 275 天（平均在分娩前 5.4 天）和分娩时从 10 头奶牛身上采集了宫颈的连续活检样本。使用实时聚合酶链反应测定白细胞介素（IL）-1β、IL-6、IL-8、IL-10和肿瘤坏死因子（TNF）α的信使RNA表达水平，并通过Luna和Sirius Red染色估计中性粒细胞和嗜酸性粒细胞的数量。分娩时，与妊娠185天相比，IL-8表达增加了430倍（p < 0.001），大量中性粒细胞侵入子宫颈，而嗜酸性粒细胞仍然稀少，IL-1β增加了8倍（p < 0.05），IL-6没有显着变化。此外，与妊娠第 185 天相比，IL-10 增加了 10 倍（p < 0.001），TNFα 减少了 57%（p < 0.05）。 IL-8 表达的大幅增加使中性粒细胞流入，表明其在宫颈成熟的最后阶段和分娩时发挥重要作用。先前的研究表明，中性粒细胞分泌基质金属蛋白酶（MMP），这可能有助于宫颈软化。相比之下，IL-1 水平仅略有增加，IL-6 浓度稳定，TNFα 下降，IL-10 表达增加的潜在后果表明，足月分娩时牛的最终宫颈成熟是一个受调节细胞因子影响的炎症过程。
The final stages of cervical ripening and parturition resemble an inflammatory process. Although the role of cytokines in both spontaneous and experimentally induced parturitions has been described in several small laboratory animals and humans, the involvement of pro-inflammatory and regulatory cytokines in physiologic parturition in cows has not been determined. In this study, the cytokine expression profiles were assessed in bovine cervical tissue at several stages of pregnancy and at parturition. Serial biopsy samples of the cervix were obtained from 10 cows on day 185 and day 275 of pregnancy (which was on average 5.4 days before parturition) and at parturition. Messenger RNA expression levels of interleukin (IL)-1beta, IL-6, IL-8, IL-10 and tumour necrosis factor (TNF)alpha were determined using real-time polymerase chain reaction and the number of neutrophils and eosinophils was estimated by Luna and Sirius Red staining. At parturition, IL-8 expression had increased 430-fold (p < 0.001) when compared with that of the day 185 of pregnancy, large numbers of neutrophils had invaded the cervix while eosinophils remained scarce, IL-1beta had increased eightfold (p < 0.05) and IL-6 had not changed significantly. Additionally, IL-10 was increased by 10-fold (p < 0.001) and TNFalpha decreased by 57% (p < 0.05) when compared with that of the day 185 of pregnancy. The large increase in expression of IL-8, enabling the influx of neutrophils, is indicative of its important role in the final stage of cervical ripening and at parturition. As previous studies have shown that neutrophils excrete matrix metalloproteinases (MMP), this might contribute to softening of the cervix. In contrast, the only slightly increased levels of IL-1, steady concentrations of IL-6 and decreased TNFalpha, the potential consequences of increased IL-10 expression, indicate that final cervical of cows in ripening at term parturition is an inflammatory process influenced by regulatory cytokines.
DOI：
10.1073/pnas.94.4.1344
发表时间：
1997-02-18
影响因子：
11.1
作者：
Mehlhop, PD;vandeRijn, M;Oettgen, HC
通讯作者：
Oettgen, HC
影响因子：
6
作者：
Fujimoto, T;Savani, RC;Strauss, JF
通讯作者：
Strauss, JF
影响因子：
9.8
作者：
Hassan, Sonia S.;Romero, Roberto;Malone, John, Jr.
通讯作者：
Malone, John, Jr.