Gallyas Silver Impregnation of Myelinated Nerve Fibers
Gallyas Silver Impregnation of Myelinated Nerve Fibers
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DOI：
10.21769/bioprotoc.3436
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发表时间：
2019-11-20
期刊：
影响因子：
0.8
通讯作者：
Stegmueller, Judith
中科院分区：
文献类型：
--
作者：
Joseph, Sabitha;Werner, Hauke B.;Stegmueller, Judith
在脊椎动物的神经系统中，神经冲动的传播由于神经轴突被髓鞘包裹而加速。髓鞘是外周神经系统雪旺细胞和中枢神经系统少突胶质细胞的分子特化、富含脂质的质膜延伸。为了可视化有髓神经纤维，并允许对大脑和脊髓中的髓磷脂进行形态学分析，Ferenc Gallyas于1979年最初开发了髓磷脂银浸染的有效方法，称为Gallyas银浸染。Gallyas的方法是基于髓磷脂的亲银特性来形成和结合银颗粒，而这一过程在髓磷脂以外的组织中受到抑制。银颗粒最终在显影步骤（“物理显影剂”）中增强。这种方法的主要优点是，它有效地可视化大髓鞘纤维束和单个髓鞘轴突。在此，我们根据Gallyas的原始方案和Pistorio及其同事随后的修改，提供了适用于石蜡包埋切片和光学显微镜使用的实验室方案。
In the nervous system of vertebrates, nerve impulse propagation is accelerated by the ensheathment of neuronal axons with myelin. Myelin sheaths are molecularly specialized, lipid-rich plasma membrane extensions of Schwann cells in the peripheral nervous system and oligodendrocytes in the central nervous system (CNS). To visualize myelinated nerve fibers and to allow for the morphological analyses of myelin in the brain and the spinal cord, an efficient method for silver impregnation of myelin has originally been developed by Ferenc Gallyas in 1979, referred to as Gallyas silver impregnation. Gallyas' method is based on the agyrophilic characteristic of myelin to form and bind silver particles, while this process is suppressed in tissues other than myelin. The silver particles are finally enhanced in a developing step ("physical developer"). The main advantage of this method is that it efficiently visualizes both large myelinated fiber tracts and individual myelinated axons. Here we provide our laboratory protocol that is suitable for paraffin embedded sections and the use of light microscopy based on Gallyas' original protocol and subsequent modifications by Pistorio and colleagues.