TSLP-induced placental DC activation and IL-10+ NK cell expansion:: Comparative study based on BALB/cxC57BL/6 and NOD/SCIDxC57BL/6 pregnant models
TSLP-induced placental DC activation and IL-10+ NK cell expansion:: Comparative study based on BALB/cxC57BL/6 and NOD/SCIDxC57BL/6 pregnant models
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DOI：
10.1016/j.clim.2007.09.006
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发表时间：
2008-01-01
影响因子：
8.6
通讯作者：
Saito, Shigeru
中科院分区：
文献类型：
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作者：
Lin, Yi;Zhong, Yanmin;Saito, Shigeru
胸腺基质淋巴细胞生成素 (TSLP)-TSLP-R 相互作用激活 CD11c(+) 树突状细胞 (DC) 并增加上皮细胞。细胞 Th2 型细胞因子的产生。我们在妊娠第 12.5 天检测了妊娠 BALB/cxC57BL/6 和 NOD/SCIDxC57BL/6 小鼠的 CK7(+) 滋养层细胞上的细胞内 TSLP 表达和胎盘 DC 上的 TSLP-R 表达。鼠重组 TSLP 激活 BALB/c 小鼠的 DC，CD80 和 CD83 表达增加； TSLP 激活的 DC 诱导产生 IL-10 的 NK 细胞扩增。这种现象可以通过抗 TSLP Ab 或单独培养 CD49b(+) NK 细胞来消除。当DC和CD49b(+)细胞单独培养时，没有发生TSLP-DC诱导的IL-10(+)CD49b(+)细胞扩增。尽管 TSLP 诱导的 DC 激活发生在 NOD/SCID 小鼠中，但 IL-10(+) NK 细胞百分比没有变化。 CK7(+) 滋养层细胞可能通过 TSLP-TSLP-R 相互作用激活胎盘 DC，并诱导 DC 依赖性胎盘 NK 细胞产生 IL-10。 TSLP-DC 和 NK 细胞接触对于 IL-10(+)CD49b(+) 细胞扩增是必需的。胎盘。来自 NOD/SCIDxC57BL/6 小鼠的 NK 细胞似乎对 TSLP-DC 诱导不太敏感。 (C) 2007 Elsevier Inc. 保留所有权利。
Thymic stromal Lymphopoietin (TSLP)-TSLP receptor (TSLP-R) interactions activate CD11c(+) dendritic cells (DCs) and increase epithelial. cell Th2-type cytokine production. We detected intracellular TSLP expression on CK7(+) trophoblast cells and TSLP-R expression on placental DCs from pregnant BALB/cxC57BL/6 and NOD/SCIDxC57BL/6 mice on gestational day 12.5. Murine recombinant TSLP activated DCs from BALB/c mice, with increased CD80 and CD83 expressions; TSLP-activated DCs induced IL-10-producing NK cell expansion. This was abrogated by anti-TSLP Ab or by culturing CD49b(+) NK cells alone. No TSLP-DC-induced IL-10(+)CD49b(+) cell expansion occurred when DCs and CD49b(+) cells were cultured separately. Although TSLP-induced DC activation occurred in NOD/SCID mice, the IL-10(+) NK cell percentage was unchanged. CK7(+) trophoblast cells may activate placental DCs via a TSLP-TSLP-R interaction and induce DC-dependent placental NK cell IL-10 production. TSLP-DC and NK cell contact appears necessary for IL-10(+)CD49b(+) cell expansion. Placental. NK cells from NOD/SCIDxC57BL/6 mice appear less sensitive to TSLP-DC induction. (C) 2007 Elsevier Inc. All rights reserved.