Role of Pitx1 upstream of Tbx4 in specification of hindlimb identity
Role of Pitx1 upstream of Tbx4 in specification of hindlimb identity
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DOI：
10.1126/science.283.5408.1736
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发表时间：
1999-03-12
期刊：
影响因子：
56.9
通讯作者：
Tabin, CJ
中科院分区：
文献类型：
--
作者：
Logan, M;Tabin, CJ
尽管最近在理解肢体模式方面取得了突破，但决定前肢和后肢之间差异的遗传因素尚不清楚。 Pitx1 和 Tbx4 基因编码的转录因子在整个后肢发育过程中表达，但在前肢芽中不表达。鸡翅芽中 Pitx1 的错误表达诱导 Tbx4 以及 HoxC10 和 HoxC11 的远端表达，这些表达通常仅限于后肢表达域。 Pitx1错误表达的翼芽发育成具有后肢某些形态特征的四肢：弯曲度改变为通常在腿中观察到的，手指的相对大小和形状更像脚趾，肌肉模式转变为腿的肌肉模式。
In spite of recent breakthroughs in understanding Limb patterning, the genetic factors determining the differences between the forelimb and the hindlimb have not been understood. The genes Pitx1 and Tbx4 encode transcription factors that are expressed throughout the developing hindlimb but not forelimb buds. Misexpression of Pitx1 in the chick wing bud induced distal expression of Tbx4, as well as HoxC10 and HoxC11, which are normally restricted to hindlimb expression domains. Wing buds in which Pitx1 was misexpressed developed into Limbs with some morphological characteristics of hindlimbs: the flexure was altered to that normally observed in legs, the digits were more toe-like in their relative size and shape, and the muscle pattern was transformed to that of a Leg.