Non-destructive sex-specific monitoring of early embryonic growth rate in light brown broiler eggs using light transmission and its correlation with hatching time and chick weight. British Poultry Science
Non-destructive sex-specific monitoring of early embryonic growth rate in light brown broiler eggs using light transmission and its correlation with hatching time and chick weight. British Poultry Science
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利用光传输对浅棕色肉鸡蛋早期胚胎生长速率进行非破坏性性别特异性监测及其与孵化时间和雏鸡体重的相关性。
DOI：
10.1080/00071668.2020.1820952
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发表时间：
2020
影响因子：
2
通讯作者：
N.
中科院分区：
文献类型：
--
作者：
Rahman;A.;Syduzzaman;M.;Khaliduzzaman;A.;Fujitani;S.;Kashimori;A.;Suzuki;T.;Ogawa;Y.;Kondo;N.
1. 监测早期胚胎生长速率具有显著的经济效益和动物福利效益。本研究的重点是利用光透射技术监测性别特异性的早期EGR，并将其与孵化时间和雏鸟体重相关联。特别是对于肉鸡蛋，浅棕色蛋壳的光谱掩蔽需要解决。这是利用纵向可见透射光谱结合蛋壳彩色图像分析完成的。在孵育之前，捕获鸡蛋的彩色图像，然后从孵育第1天到第9天每天测量鸡蛋的透射光谱。卵的性别随后在孵化2天后被证实。为了准确灵敏地利用光透射法测定EGR的性别差异，同时最大限度地减少蛋壳颜色和厚度的干扰，确定纵向透射比在575 nm和610 nm时最有效。胚胎生长可在孵育第3天（72小时）检测到，比先前报道的横向传播测量早24小时。然而，在这个时候，低血水平意味着T575/T610的平均比率没有显著的性别差异（P >.05）。这可能部分是由于浅棕色蛋壳的光谱掩盖。第7 d时，雌性胚胎的T575/T610比值显著低于雄性（P < 0.05）。虽然T575/T610比例与小鸡孵化时间和孵化重的相关性较低，但这可能是进一步进行此类预测的非破坏性调查的良好起点。综上所述，该方法对区分不同性别的肉鸡蛋早期EGR具有较高的敏感性，具有推进精准孵化管理和家禽研究的潜力。
1. Monitoring early embryonic growth rate (EGR) has significant economic and animal welfare benefits. This study focuses on monitoring sex-specific early EGR using light transmission, and correlating this with hatching time and chick weight. For broiler eggs in particular, spectral masking of the light brown eggshells needed to be addressed. This was done using longitudinal visible transmission spectroscopy combined with eggshell colour image analysis.2. Prior to incubation, colour images of eggs were captured followed by daily measurements of transmission spectra of eggs from days one to nine of incubation. The sex of the eggs was subsequently verified 2 d after hatching.3. To accurately and sensitively determine sex differences in EGR using light transmission, while minimising interference from eggshell colour and thickness, the ratio of longitudinal transmissions was determined to be most effective at 575 and 610 nm.3. Embryonic growth was detectable from d 3 (72 h) of incubation, 24 h earlier than previously reported lateral transmission measurements. However, at this time, low blood levels meant that no significant sex-differences (P > 0.05) for the mean T575/T610 ratio were detectable. This may have been due, in part, to spectral masking from the light brown eggshells. At d 7, female embryos had a significantly lower (P < 0.05) mean T575/T610 ratio than males.4. Although the T575/T610 ratio had low correlations with hatching time and hatch-weight of chicks, this could be a good starting point for further non-destructive investigations for such predictions.5. In conclusion, the methodology had the sensitivity to differentiate sex-specific early EGR in broiler eggs, even with pigmented eggshells, and has the potential to advance precision hatchery management and poultry research.