EPITOPE MAPPING OF FOOT-AND-MOUTH-DISEASE VIRUS WITH NEUTRALIZING MONOCLONAL-ANTIBODIES
EPITOPE MAPPING OF FOOT-AND-MOUTH-DISEASE VIRUS WITH NEUTRALIZING MONOCLONAL-ANTIBODIES
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DOI：
10.1099/0022-1317-70-1-59
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发表时间：
1989-01-01
影响因子：
3.8
通讯作者：
ROWLANDS, DJ
中科院分区：
文献类型：
--
作者：
BOLWELL, C;CLARKE, BE;ROWLANDS, DJ
用单抗对口蹄疫病毒A22伊拉克24/株的表位进行了定位。使用了三种方法：(I)使用一组重叠的多肽的间接ELISA法，(Ii)针对每个单抗产生中和逃逸变异体，以及(Iii)中和逃逸变异体的测序。研究表明，该病毒在VP1上的一个主要抗原部位至少有三个重叠的线性中和表位。第二个构象位点的存在被证明，但它在病毒颗粒上的位置没有被定位。带有代表主要部位的序列的合成肽可诱导抗体，这些抗体与多克隆血清或与病毒产生的针对一系列野外分离株的中和单抗具有类似的广泛交叉中和活性。
Epitopes of strain A22 Iraq 24/64 of foot-and-mouth disease virus have been mapped with monoclonal antibodies (MAbs). Three methods were used: (i) an indirect ELISA using an overlapping set of peptides, (ii) production of neutralization escape variants against each MAb and (iii) sequencing of neutralization escape variants. The study has shown that the virus has at least three overlapping linear neutralizing epitopes within a major antigenic site on VP1. The presence of a second, conformational site was demonstrated but its position on the virus particle was not located. Synthetic peptides with sequences representing the major site elicit antibodies which have similar broad cross-neutralizing activity to polyclonal serum or neutralizing MAbs produced with the virus against a range of field isolates.