Preferential induction of Th17 cells in vitro and in vivo by Fucogalactan from Ganoderma lucidum (Reishi).
Preferential induction of Th17 cells in vitro and in vivo by Fucogalactan from Ganoderma lucidum (Reishi).
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DOI：
10.1016/j.bbrc.2012.04.135
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发表时间：
2012-05
影响因子：
3.1
通讯作者：
H. Yoshida;Mayu Suzuki;Ryota Sakaguchi;I. Tani;H. Kotani;N. Shudo;A. Yoshimura
中科院分区：
文献类型：
--
作者：
H. Yoshida;Mayu Suzuki;Ryota Sakaguchi;I. Tani;H. Kotani;N. Shudo;A. Yoshimura
被称为灵芝（灵芝）的蘑菇已被用作治疗肿瘤和激活免疫系统的草药。由于其对效应T辅助细胞分化的影响尚未完全了解，我们研究了灵芝及其主要成分β-葡聚糖对树突状细胞活化和Th 17细胞分化的影响。灵芝提取物以及纯化的β-葡聚糖（Curdran）激活DC并使它们产生大量IL-23。β-葡聚糖还增强并维持IL-23 p19的转录。MEK-ERK信号通路正向调节β-葡聚糖刺激的DC中IL-23 p19的转录。在混合白细胞反应中，灵芝刺激的DC优先诱导Th 17细胞。此外，口服灵芝增加了Th 17细胞的百分比和抗菌肽的转录水平。我们的研究结果表明，灵芝和β-葡聚糖激活DC产生大量的IL-23，其在体外和体内均诱导Th 17分化。
The mushroom known as Reishi (Ganoderma lucidum) has been used as an herbal medicine for tumor treatment and immune system activation. Because its effects on the differentiation of effector T helper cells have not yet been fully understood, we investigated the effects of Reishi and those of its principal ingredient, β-glucan, on the activation of dendritic cells and the differentiation of Th17 cells. Reishi extracts as well as purified β-glucan (Curdran) activated DCs and caused them to produce large amounts of IL-23. β-glucan also enhanced and sustained the transcription of IL-23p19. The MEK-ERK signaling pathway positively regulates IL-23p19 transcription in β-glucan-stimulated DCs. In a mixed leukocyte reaction, Reishi-stimulated DCs preferentially induced Th17 cells. Furthermore, orally-administrated Reishi increased the percentages of Th17 cells and the transcription levels of antimicrobial peptides. Our results show that Reishi and β-glucan activate DCs to produce large amounts of IL-23, which induces Th17 differentiation both in vitro and in vivo.