The renaissance of bacillosamine and its derivatives: pathway characterization and implications in pathogenicity.
The renaissance of bacillosamine and its derivatives: pathway characterization and implications in pathogenicity.
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DOI：
10.1021/bi401546r
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发表时间：
2014-02-04
期刊：
影响因子：
2.9
通讯作者：
Imperiali B
中科院分区：
文献类型：
--
作者：
Morrison MJ;Imperiali B
原核生物特异性糖，包括N，N ' -二乙酰杆菌胺（diNAcBac）和假氨基酸，在过去十年中经历了复兴，因为它们在与微生物致病性相关的聚糖中被发现。DiNAcBac位于革兰氏阴性病原体（包括空肠弯曲杆菌和淋病奈瑟菌）的N-和o -连锁细菌蛋白糖基化途径的低聚糖还原端。diNAcBac的进一步衍生化产生了军团胺酸（legionaminic acid），它首先在嗜肺军团菌脂多糖（LPS）的o抗原中被表征。假氨基酸是军团胺酸的一种异构体，在幽门螺杆菌等致病菌中也很重要，因为它存在于鞭毛蛋白的o -链糖基化中，在鞭毛组装和运动中起重要作用。在这里，我们介绍了导致这些高度修饰的糖的生物合成途径的表征以及每种糖在细菌适应性和致病性中所起作用的研究的最新进展。
Prokaryote-specific sugars, including N,N′-diacetylbacillosamine (diNAcBac) and pseudaminic acid, have experienced a renaissance in the past decade because of their discovery in glycans related to microbial pathogenicity. DiNAcBac is found at the reducing end of oligosaccharides of N- and O-linked bacterial protein glycosylation pathways of Gram-negative pathogens, including Campylobacter jejuni and Neisseria gonorrhoeae. Further derivatization of diNAcBac results in the nonulosonic acid known as legionaminic acid, which was first characterized in the O-antigen of the lipopolysaccharide (LPS) in Legionella pneumophila. Pseudaminic acid, an isomer of legionaminic acid, is also important in pathogenic bacteria such as Helicobacter pylori because of its occurrence in O-linked glycosylation of flagellin proteins, which plays an important role in flagellar assembly and motility. Here, we present recent advances in the characterization of the biosynthetic pathways leading to these highly modified sugars and investigation of the roles that each plays in bacterial fitness and pathogenicity.