Cyanine-labeling reagents: Sulfobenzindocyanine succinimidyl esters
Cyanine-labeling reagents: Sulfobenzindocyanine succinimidyl esters
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DOI：
10.1021/bc960021b
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发表时间：
1996-05-01
影响因子：
4.7
通讯作者：
Waggoner, AS
中科院分区：
文献类型：
--
作者：
Mujumdar, SR;Mujumdar, RB;Waggoner, AS
本文报道了一种使花青染料标记物的吸收和发射波长向红光方向移动15 - 30 nm的合成方法。该步骤显著增加了制备用于细胞计数和诊断中的多参数分析的荧光探针的潜力。新的磺基苯并吲哚菁染料含有琥珀酰亚胺酯作为反应基团，可以很容易地结合到抗体，抗生物素蛋白，修饰的DNA，和其他含氨基的材料。标记试剂是水溶性的，并且它们的荧光对pH不敏感。其中一种试剂Cy3.205可以用568 nm Kr激光线最佳激发，并且可用于共聚焦显微镜和流式细胞术。另一种染料Cy5.205可以用633 He-Ne或647 nm Kr激光线激发，这些激光线可用于许多流式细胞仪和激光扫描显微镜。在660 - 690 nm附近发射的新激光二极管也应该是Cy5.205的优良激发源。
A synthetic method for shifting the absorption and emission wavelengths of cyanine dye labels by 15-30 nm to the red has been developed. This step significantly increases the potential for preparing fluorescent probes for multiparameter analysis in cytometry and diagnostics. The new sulfobenzindocyanine dyes contain succinimidyl esters as reactive groups and can be readily conjugated to antibodies, avidin, modified DNA, and other amino group-containing materials. The labeling reagents are water soluble, and their fluorescence is not sensitive to pH. One of the reagents, Cy3.205, can be optimally excited with the 568 nm Kr laser line and is useful for confocal microscopy and flow cytometry. Another dye, Cy5.205, can be excited with the 633 He-Ne or 647 nm Kr laser lines available with many flow cytometers and laser-scanning microscopes. New laser diodes emitting near 660-690 nm should also be excellent excitation sources for Cy5.205.