Screening of pure synthetic coating substrates for induced pluripotent stem cells and iPSC-derived neuroepithelial progenitors with short peptide based integrin array
Screening of pure synthetic coating substrates for induced pluripotent stem cells and iPSC-derived neuroepithelial progenitors with short peptide based integrin array
复制标题
使用基于短肽的整合素阵列筛选诱导多能干细胞和 iPSC 衍生的神经上皮祖细胞的纯合成涂层基质
DOI：
10.1016/j.yexcr.2019.04.013
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发表时间：
2019-07-01
影响因子：
3.7
通讯作者：
Wen, Xuejun
中科院分区：
文献类型：
--
作者：
Jiang, Chenyang;Zeng, Xiaomei;Wen, Xuejun
在干细胞研究中，需要简单、纯净的合成涂层基质来克服传统涂层产品（如动物源性基质胶）的缺点。由于整合素在细胞粘附和细胞 ECM 通讯中非常重要，因此在本研究中，使用由合成整合素结合肽建立的市售整合素阵列来筛选 iPSC 和 NEP 的涂层基质。结果表明，整合素α（5）β（1）、α（v）β（1）、α（M）β（2）和α（IIb）β（3）结合肽支持iPSC的细胞粘附，而α（5）β（1）、α（v）β（1）和α（IIb）β（3）结合肽支持NEP粘附。此外，整合素 α(5)β(1) 结合肽可支持 iPSC 和 iPSC 衍生的 NEP 的快速扩增以及 NEP 的生成过程，其效率与 Matrigel 相同。在这项工作中，我们证明，通过以整合素依赖性方式支持干细胞生长，整合素阵列和涂层系统有潜力在神经疾病建模中开发更精确和有效的系统。
Simple and pure synthetic coating substrates are needed to overcome the disadvantages of traditional coating products like animal derived Matrigel in stem cell research. Since integrins are of great importance in cell adhesion and cell-ECM communication, in this study, a commercially available integrin array established by synthetic integrin binding peptides is used to screen coating substrates for iPSCs and NEPs. The results showed that binding peptides of integrin alpha(5)beta(1), alpha(v)beta(1), alpha(M)beta(2) and alpha(IIb)beta(3) supported cell adhesion of iPSCs, while alpha(5)beta(1), alpha(v)beta(1) and alpha(IIb)beta(3) binding peptides supported NEPs adhesion. Additionally, integrin alpha(5)beta(1) binding peptide was revealed to support rapid expansion of iPSCs and iPSC-derived NEPs, as well as the process of NEPs generation, with equal efficiency as Matrigel. In this work, we demonstrated that by supporting stem cell growth in an integrin dependent manner, the integrin array and coating system has the potential to develop more precise and efficient systems in neurological disease modeling.