Induction of Spontaneous Liposome Adsorption by Exogenous Surface Modification with Cell-Penetrating Peptide-Conjugated Lipids
Induction of Spontaneous Liposome Adsorption by Exogenous Surface Modification with Cell-Penetrating Peptide-Conjugated Lipids
复制标题
DOI：
10.1021/acs.langmuir.1c01072
复制
发表时间：
2021-08-03
期刊：
影响因子：
3.9
通讯作者：
Teramura, Yuji
中科院分区：
文献类型：
--
作者：
Sato, Yuya;Asawa, Kenta;Teramura, Yuji
使用两亲性分子，例如聚乙二醇缀合磷脂（PEG-脂质），可以通过与脂质自组装的外源添加而掺入脂质体表面。该技术可适用于脂质体和细胞的操作。在本研究中，我们旨在表征用于外源表面修饰脂质体（尺寸：约 100 nm）的 Tat 肽 (YGRKKRRQRRR) 缀合 PEG-脂质。我们之前报道过，用与 PEG-脂质缀合的 Tat 肽进行表面修饰的细胞可以自发地附着在材料表面，无需任何化学修饰。在这里，我们通过将不同分子量（5和40 kDa）的PEG与具有三个酰基链（分别为肉豆蔻酰基、棕榈酰基和硬脂酰基）的不同脂质组合来合成不同类型的Tat-PEG-脂质，然后研究由不同Tat-PEG-脂质诱导的修饰脂质体在基质表面上的自发吸附。吸附的脂质体的量很大程度上取决于掺入的 Tat-PEG-脂质部分的数量：PEG 和酰基链长度的减少导致吸附更高量的脂质体。此外，当胶原酶可裂解的氨基酸序列插入Tat序列和PEG片段之间时，可以通过胶原酶处理从基质中收获吸附的脂质体，并且不同Tat-PEG-脂质之间的解吸效率没有差异。因此，Tat-PEG-脂质可以成为操纵脂质体和细胞的合适工具。
The use of amphiphilic molecules such as poly(ethylene glycol)-conjugated phospholipid (PEG-lipid) enables incorporation into liposome surfaces by exogenous addition as a result of the self-assembly with lipids. This technique can be applicable for manipulation of both liposomes and cells. In this study, we aimed to characterize Tat peptide (YGRKKRRQRRR)-conjugated PEG-lipids when used to exogenously surface modify liposomes (size: ca. 100 nm). We earlier reported that cells, which were surface modified with Tat peptides conjugated to PEG-lipids could attach spontaneously to material surfaces without any chemical modification. Here, we synthesized different types of Tat-PEG-lipids by combining PEG of different molecular weights (5 and 40 kDa) with different lipids with three acyl chains (myristoyl, palmitoyl, and stearoyl, respectively) and then studied the spontaneous adsorption of modified liposomes onto a substrate surface induced by the different Tat-PEG-lipids. The amount of adsorbed liposomes strongly depended on the number of incorporated Tat-PEG-lipid moieties: a decrease in both the PEG and the acyl chain lengths led to adsorption of higher amounts of liposomes. Furthermore, when a collagenase-cleavable amino acid sequence was inserted between the Tat sequence and the PEG segment, adsorbed liposomes could be harvested from the substrate by collagenase treatment with no difference in desorption efficiency between the different Tat-PEG-lipids. Thus, Tat-PEG-lipid can be a suitable tool for the manipulation of liposomes and cells.