Preparation of PLLA membranes with different morphologies for culture of MG-63 Cells
Preparation of PLLA membranes with different morphologies for culture of MG-63 Cells
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DOI：
10.1016/j.biomaterials.2003.10.098
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Young, TH
中科院分区：
文献类型：
--
作者：
Liu, HC;Lee, IC;Young, TH
本工作制备了不同形貌的聚L-乳酸（PLLA）膜，研究了乙醇、二氯甲烷和PLLA成膜体系的平衡相图。根据相图，通过改变铸膜液中PLLA的浓度，可以分别制备出以结晶为主的颗粒膜和以液-液分层为主的多孔膜。此外，MG-63骨肉瘤细胞和PLLA膜的致密，多孔和颗粒形态的体外相互作用进行了研究。结果发现，微粒膜不仅可以促进细胞的粘附和生长，而且可以上调成骨细胞的表型。因此，具有颗粒形态的PLLA膜满足了用于移植成骨细胞的临时支架所必需的生物材料要求，并且为更复杂的组织工程系统的架构设计提供了手段。(C)2003爱思唯尔有限公司。保留所有权利。
In this work, Poly L-lactide (PLLA) membranes with different morphologies were prepared and the equilibrium phase diagram of membrane formation system of ethanol, methylene chloride, and PLLA was studied. Based on the phase diagram, particulate and porous membranes, dominated by crystallization and liquid-liquid demixing, respectively, could be prepared by changing the PLLA concentration of casting solution. In addition, in vitro interaction of MG-63 osteosarcoma cells and PLLA membranes with dense, porous and particulate morphologies was investigated. It was found that the particulate membrane not only could improve cell adhesion and growth, but also could upregulate the osteoblastic phenotype. Therefore, the PLLA membrane with particulate morphology satisfies the biomaterial requirement necessary for temporary scaffold to transplanted osteoblasts and provides a means for the architectural design of more complex tissue-engineered systems. (C) 2003 Elsevier Ltd. All rights reserved.