Firefly luciferase exhibits bimodal action depending on the luciferin chirality
Firefly luciferase exhibits bimodal action depending on the luciferin chirality
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DOI：
10.1016/j.bbrc.2005.03.202
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发表时间：
2005-06-03
影响因子：
3.1
通讯作者：
Niwa, H
中科院分区：
文献类型：
--
作者：
Nakamura, M;Maki, S;Niwa, H
即使在普通的有氧荧光素-荧光素酶反应条件下，萤火虫荧光素酶也能够将(L)-荧光素转化为荧光素酰辅酶A。荧光素酶能够严格识别荧光素结构的手性，充当(L)-荧光素的酰基辅酶A合成酶，而(D)-荧光素用于生物发光反应。 (D)-荧光素抑制 (L)-荧光素的荧光素辅酶A合成酶活性，而 (L)-荧光素延迟 (D)-荧光素的生物发光反应，这意味着这两种酶活性均被其自身底物的对映异构体阻止。 (c) 2005 Elsevier Inc. 保留所有权利。
Firefly luciferase is able to convert (L)-luciferin into luciferyl-CoA even under ordinary aerobic luciferin-luciferase reaction conditions. The luciferase is able to recognize strictly the chirality of the luciferin structure, serving as the acyl-CoA synthetase for (L)-luciferin, whereas (D)-luciferin is used for the bioluminescence reaction. (D)-Luciferin inhibits the luciferyl-CoA synthetase activity Of (L)-luciferin, whereas (L)-luciferin retards the bioluminescence reaction Of (D)-luciferin, meaning that both enzyme activities are prevented by the enantiomer of its own substrate. (c) 2005 Elsevier Inc. All rights reserved.