Expression and localization of human herpesvirus 8-encoded proteins in primary effusion lymphoma, Kaposi's sarcoma, and multicentric Castleman's disease
Expression and localization of human herpesvirus 8-encoded proteins in primary effusion lymphoma, Kaposi's sarcoma, and multicentric Castleman's disease
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DOI：
10.1006/viro.2000.0196
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发表时间：
2000-04-10
期刊：
影响因子：
3.7
通讯作者：
Sata, T
中科院分区：
文献类型：
--
作者：
Katano, H;Sato, Y;Sata, T
为研究人疱疹病毒8型（HHV 8）编码蛋白在原发性渗出性淋巴瘤（PEL）、卡波西肉瘤（KS）和多中心性Castleman病（MCD）细胞中的表达，制备了9株兔抗人疱疹病毒8型（HHV 8）编码蛋白K2、ORF26、Ks、K8.1、K10、K11、ORF59、ORF65和ORF73的多克隆抗体。Western blot分析显示，除ORF73（拉娜）外，其余蛋白质的表达均被TPA诱导，表明这些蛋白质是裂解蛋白。免疫荧光分析在原代PEL细胞来自心包积液和PEL细胞系与TPA治疗和不显示，原代PEL细胞表现出相同的表达模式，作为非诱导的PEL细胞系，和治疗改变的Ks，ORF59和ORF65蛋白的定位。免疫组化显示，90%的KS梭形细胞表达ORF73蛋白，而一小部分KS细胞表达Ks、K10、K11、ORF59和ORF65蛋白。在MCD中，ORF73、ORF59、Ks、K2和K10蛋白在卵泡套层细胞中表达。这些数据表明，KS和PEL细胞主要表达潜伏蛋白，而MCD表达潜伏蛋白和裂解蛋白，这表明HHV 8在HHV 8相关疾病的发病机制中起着不同的作用。(C)北京大学出版社.
To investigate the expression of human herpesvirus 8 (HHV8)-encoded proteins in the cells of primary effusion lymphoma (PEL), Kaposi's sarcoma (KS) and multicentric Castleman's disease (MCD), nine rabbit polyclonal antibodies to K2, ORF26, Ks, K8.1, K10, K11, ORF59, ORF65, and ORF73 were developed. Western blot analysis in PEL cell lines (TY-1 and BCBL-1) revealed that the expression of these proteins, except ORF73 (LANA), was induced by tetradecanoylphorbol acetate (TPA) treatment, indicating that these proteins are lytic proteins. Immunofluorescence assay in primary PEL cells derived from pericardial effusion and PEL cell lines with and without TPA treatment revealed that primary PEL cells exhibited the same expression pattern as noninduced PEL cell lines, and the treatment changed localization of Ks, ORF59, and ORF65 proteins. Immunohistochemistry revealed that 90% of KS spindle cells expressed the ORF73 protein, whereas a small population of KS cells expressed Ks, K10, K11, ORF59, and ORF65 proteins. In MCD, ORF73, ORF59, Ks, K2, and K10 proteins were expressed in the cells at mantle zone of the follicle. These data indicate that KS and PEL cells expressed predominantly latent proteins, whereas MCD expressed both latent and lytic proteins, suggesting that HHV8 plays a different role in the pathogenesis of HHV8-associated diseases. (C) 2000 Academic Press.