Expression of leukocyte adhesion molecules in human endometrium.
Expression of leukocyte adhesion molecules in human endometrium.
复制标题
人子宫内膜中白细胞粘附分子的表达。
DOI：
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发表时间：
1990
影响因子：
3.5
通讯作者：
Demetrio Poubouridis
中科院分区：
文献类型：
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作者：
S. Tabibzadeh;Demetrio Poubouridis
在本研究中，研究了参与白细胞结合的分子在人子宫内膜中的分布。用单克隆抗体ABC法检测了13例增殖期子宫内膜、10例分泌期子宫内膜、子宫内膜腺体和间质细胞间粘附分子-1（ICAM-1）、淋巴细胞功能相关抗原（LFA-1）和HLA-DR分子的表达。ICAM-1和HLA-DR分子在淋巴细胞和内皮细胞中均呈强表达。ICAM-1在上皮细胞中表达均匀，而HLA-DR分子在基底层的上皮细胞中优先表达。上皮细胞HLA-DR和ICAM-1分子的表达增强邻近淋巴聚集体。ICAM-1分子在基底层和功能层的基质细胞中均匀表达，而HLA-DR分子仅在淋巴样细胞周围的基质细胞中表达。ICAM-1在上皮细胞和基质细胞中的表达通过免疫组织化学在分离的完整基质细胞和腺体中证实。虽然在体外增殖的基质和上皮细胞表达ICAM-1，他们很少HLA-DR阳性。LFA-1的表达局限于淋巴细胞。ICAM-1、LFA-1和HLA-DR分子在人子宫内膜成分中的高水平表达可能有助于淋巴细胞在人子宫内膜中的存在、聚集和优先分布。
In the present investigation the distribution of molecules that are involved in the leukocyte binding was studied in human endometrium. The expression of intercellular adhesion molecule-1 (ICAM-1), lymphocyte function-associated antigen (LFA-1), and HLA-DR molecules was studied in 13 proliferative and 10 secretory endometria as well as cultures of endometrial glands and stroma by avidin-biotin complex (ABC) procedure using monoclonal antibodies. The ICAM-1 and HLA-DR molecules were both strongly expressed in the lymphoid and endothelial cells. ICAM-1 expression was uniform in the epithelium, whereas the HLA-DR molecules were preferentially expressed in the epithelial cells in the basalis. Expression of both epithelial HLA-DR and ICAM-1 molecules was enhanced adjacent to lymphoid aggregates. ICAM-1 molecule was uniformly expressed in the stromal cells in the basalis and the functionalis, whereas the HLA-DR molecules were expressed exclusively in the stromal cells surrounding the lymphoid cells. ICAM-1 expression in the epithelial and stromal cells was confirmed in the isolated intact stromal cells and glands by immunohistochemistry. Although stromal and epithelial cells propagated in vitro expressed ICAM-1, they were rarely HLA-DR positive. The expression of LFA-1 was confined to the lymphoid cells. The high level of expression of ICAM-1, LFA-1, and HLA-DR molecules in human endometrial constituents may contribute to the presence, aggregation, and preferential distribution of lymphoid cells in human endometrium.