DELTA-OPIOID RECEPTOR GENE-EXPRESSION IN LYMPHOCYTES
DELTA-OPIOID RECEPTOR GENE-EXPRESSION IN LYMPHOCYTES
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DOI：
10.1006/bbrc.1994.2071
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发表时间：
1994-08-15
影响因子：
3.1
通讯作者：
CHUANG, RY
中科院分区：
文献类型：
--
作者：
CHUANG, LF;CHUANG, TK;CHUANG, RY
先前的研究已经表明，吗啡通过延迟感染细胞裂解的机制刺激猴免疫缺陷病毒（SIV）在SIV感染的人CEM x174细胞以及猴淋巴细胞中的复制（Biochem.Biophys.2005）。通信资源195：1165-1173，1993）。本研究描述了脑样阿片受体序列的CEM x174细胞和猴淋巴细胞的RNA转录本的鉴定。研究的淋巴细胞阿片受体的基因序列，包括第三个跨膜结构域和第三个胞质环表明在氨基酸组成的同源性96%的δ阿片受体在脑细胞。这种阿片受体序列在淋巴细胞中的表达是组成性的，因为它可以在盐水处理和吗啡处理的猴子以及在吗啡处理的猴子解毒后检测到。(C)1994年出版社出版。
Previous studies have shown that morphine stimulates simian immunodeficiency virus (SIV) replication in SIV-infected human CEM x174 cells as well as in monkey lymphocytes through a mechanism of delaying the lysis of infected cells (Biochem. Biophys. Res. Commun. 195:1165-1173, 1993). The present study describes the identification of brain-like opioid receptor sequences in RNA transcripts of both CEM x174 cells and monkey lymphocytes. Study of the gene sequence of a lymphocyte opioid receptor encompassing the third transmembrane domain and the third cytoplasmic loop indicates a 96% homology in amino acid composition to the delta opioid receptor in brain cells. Expression of such an opioid receptor sequence in,lymphocytic cells is constitutive, since it could be detected in both saline-treated and morphine-treated monkeys as well as in morphine-treated monkeys after detoxification. (C) 1994 Academic Press, Inc.