Development of chimeric receptor activator of nuclear factor‐kappa B with glutathione S‐transferase in the extracellular domain: Artificial switch in a membrane receptor
Development of chimeric receptor activator of nuclear factor‐kappa B with glutathione S‐transferase in the extracellular domain: Artificial switch in a membrane receptor
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细胞外域谷胱甘肽S-转移酶的核因子-κB嵌合受体激活剂的开发：膜受体中的人工开关
DOI：
10.1111/cbdd.14002
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发表时间：
2021
影响因子：
3
通讯作者：
Fujita Mikako
中科院分区：
文献类型：
--
作者：
Kawashima Kanako;Hirota‐Tsukimachi Mayuko;Toma Tsugumasa;Koga Ryoko;Iwamaru Kana;Kanemaru Yosuke;Yanae Misato;Ahagon Ami;Nakamura Yurine;Anraku Kensaku;Tateishi Hiroshi;Gohda Jin;Inoue Jun‐ichiro;Otsuka Masami;Fujita Mikako
已经开发了各种嵌合受体并用于生物学实验。在本研究中，我们构建了三种类型的嵌合受体激活因子核因子-κ B（RANK）与谷胱甘肽S-转移酶（GST）蛋白的细胞外结构域，并刺激他们使用新合成的化学三聚体与三个谷胱甘肽。尽管这种刺激没有激活这些蛋白质，但我们意外地发现，名为RANK-GST-SC的嵌合体（其中GST取代了RANK胞外结构域的主要部分）激活的核因子-κ B（NF-κB）信号比没有配体的野生型RANK强约6倍。细胞外GST的二聚化被认为是细胞外的开关，然后发生信号转导。GST已被广泛用作蛋白质纯化的标签; GST融合蛋白可以方便地被谷胱甘肽缀合的珠捕获，并且容易从杂质中纯化。本研究是GST的新用途的一个开创性的例子，并为新的化学生物学系统的开发提供信息。
Various chimeric receptors have been developed and used for biological experiments. In the present study, we constructed three types of chimeric receptor activator of nuclear factor‐kappa B (RANK) with the glutathione S‐transferase (GST) protein in the extracellular domain, and stimulated them using newly synthesized chemical trimerizers with three glutathiones. Although this stimulation did not activate these proteins, we unexpectedly found that the chimera named RANK‐GST‐SC, in which GST replaced a major part of the RANK extracellular domain, activated nuclear factor‐kappa B (NF‐κB) signaling approximately sixfold more strongly than wild‐type RANK without the ligand. The dimerization of extracellular GST is considered to function as a switch outside the cell, and signal transduction then occurs. GST has been widely employed as a tag for protein purification; GST‐fusion protein can be conveniently captured by glutathione‐conjugated beads and easily purified from impurity. The present study is a pioneering example of the novel utility of GST and provides information for the development of new chemical biology systems.
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含有细胞穿透肽的人工铜复合物可抑制 NF-・B 活化
影响因子：
1.7
作者：
Yosuke Kanemaru;Yumi Momiki;Saori Matsuura;Tatsufumi Horikawa;Jin Gohda;Jun-ichiro Inoue;Yoshinari Okamoto;Mikako Fujita;Masami Otuska
通讯作者：
Masami Otuska
影响因子：
5.8
作者：
Fujita, M;Akari, H;Adachi, A
通讯作者：
Adachi, A
影响因子：
64.8
作者：
Farrar, MA;AlberolaIla, J;Perlmutter, RM
通讯作者：
Perlmutter, RM
影响因子：
168.9
作者：
Rachner, Tilman D.;Khosla, Sundeep;Hofbauer, Lorenz C.
通讯作者：
Hofbauer, Lorenz C.
影响因子：
11.4
作者：
Gohda, J;Akiyama, T;Inoue, J
通讯作者：
Inoue, J