Effect of gossypol on Spisula sperm observed with real-time confocal microscopy, polarized light microscopy, and video microscopy.
Effect of gossypol on Spisula sperm observed with real-time confocal microscopy, polarized light microscopy, and video microscopy.
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通过实时共聚焦显微镜、偏光显微镜和视频显微镜观察棉酚对Spisula精子的影响。
DOI：
10.1086/bblv193n2p227
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Burgos,MH
中科院分区：
文献类型：
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作者：
Tran,PT;DePina,A;Inoué,S;Oldenbourg,R;Segal,SJ;Burgos,MH
摄入或注射棉酚抑制精子繁殖和精子活力(参考文献[1])我们在此报告了一种观察棉酚对个体精子作用的体外实验。将精子悬浮液的样本加入灌注室，灌注室由一张涂有多聚赖氨酸的盖片和一张被两条双面胶带隔开的玻璃片组成。来自双壳类软体动物的精子，S+威亚。Slidissimu，悬浮在过滤的海水溶液中；然后是0.1%的曙红-B。曙红-B仅将死精子染色为红色(2)，因此它可作为死精子的标记。精子头紧紧地粘在盖子上。通常使鞭毛在溶液中自由移动。将含有O-100 PM棉酚的过滤过的水通过小室灌流。使用实时共聚焦显微镜(3)进行观察，
Ingestion or injection of gossypol supprerser sperm produe tion and sperm motility in viro (reviewed in ref. I j. We report here an in vitro assay for observing gossypol action on individual sperm. A sample of the sperm suspension was added to a perfusion chamber consisting of a polylysine-coated coverslip and a glass slide separated by two strips of double-sticky tape. Sperm from the bivalve mollusc, S+ wia. snlidissimu, were sus-pended in a solution of filtered seaw; lter and 0.1% Eosin-B. Eosin-B stains the n~ cleuc of only dead sperm red (2), and it therefore serves as a marker Sor dead sperm. The sperm heads adhered tightly to the coverslip. often leaving the flagella free to move in solution. Filtered wnvater containing O-100 PM gossypol was perfused through the chamber. Observations were performed either with a real-time confocal microscope (3), the