Osmolarity and glucose differentially regulate aldose reductase activity in cultured mouse podocytes.
Osmolarity and glucose differentially regulate aldose reductase activity in cultured mouse podocytes.
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DOI：
10.1155/2011/278963
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发表时间：
2011
影响因子：
--
通讯作者：
Rybczyńska A
中科院分区：
文献类型：
--
作者：
Lewko B;Latawiec E;Maryn A;Barczyńska A;Pikuła M;Zieliński M;Rybczyńska A
足细胞损伤与许多肾脏疾病的进展相关，包括糖尿病肾病。在这项研究中，我们研究了是否醛糖还原酶（AR），在不同组织中的糖尿病并发症的酶牵连，是由足细胞中的高葡萄糖和渗透压调制。AR mRNA，蛋白表达和活性测定在正常和高糖和渗透压培养6小时至5天的小鼠足细胞。高渗急性刺激AR的表达和活性，随后增加AR的表达，但活性下降。高糖也升高了AR蛋白水平;然而，这并不伴随着相应的酶激活。此外，高葡萄糖似乎抵消了渗透压依赖的AR激活。总之，在足细胞AR调制高葡萄糖和增加渗透压以不同的方式。翻译后事件可能影响AR活性，与酶蛋白量无关。足细胞中AR的激活可能与糖尿病足细胞病有关。
Podocyte injury is associated with progression of many renal diseases, including diabetic nephropathy. In this study we examined whether aldose reductase (AR), the enzyme implicated in diabetic complications in different tissues, is modulated by high glucose and osmolarity in podocyte cells. AR mRNA, protein expression, and activity were measured in mouse podocytes cultured in both normal and high glucose and osmolarity for 6 hours to 5 days. Hyperosmolarity acutely stimulated AR expression and activity, with subsequent increase of AR expression but decrease of activity. High glucose also elevated AR protein level; however, this was not accompanied by respective enzyme activation. Furthermore, high glucose appeared to counteract the osmolarity-dependent activation of AR. In conclusion, in podocytes AR is modulated by high glucose and increased osmolarity in a different manner. Posttranslational events may affect AR activity independent of enzyme protein amount. Activation of AR in podocytes may be implicated in diabetic podocytopathy.
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影响因子：
--
作者：
Dan, QH;Wong, RLC;Lam, KSL
通讯作者：
Lam, KSL
DOI：
10.1152/ajprenal.2000.278.6.f999
发表时间：
2000-06-01
影响因子：
4.2
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.8
作者：
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通讯作者：
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影响因子：
19.6
作者：
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通讯作者：
Demaine, AG