Applications of silver nanoparticles capped with different functional groups as the matrix and affinity probes in surface-assisted laser desorption/ionization time-of-flight and atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry for rapid analysis of sulfur d
Applications of silver nanoparticles capped with different functional groups as the matrix and affinity probes in surface-assisted laser desorption/ionization time-of-flight and atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry for rapid analysis of sulfur d
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DOI：
10.1002/rcm.3681
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
K. Shrivas;Hui-Fen Wu
中科院分区：
文献类型：
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作者：
K. Shrivas;Hui-Fen Wu
提出了一种以不同官能团修饰的银纳米粒子（AgNPs）为基质和亲和探针，应用于表面辅助激光解吸电离飞行时间质谱（SALDI-TOF MS）和大气压基质辅助激光解吸电离离子阱质谱（AP-MALDI-ITMS）分析含硫药物和生物硫醇的方法。通过共价键合吸附在AgNPs表面上的生物硫醇受到紫外线（UV）辐射，由于NPs优异的光化学性质，能够解吸和电离。所提出的方法已成功地应用于测定半胱氨酸和同型半胱氨酸在人尿样中使用的内标。尿样中半胱氨酸和同型半胱氨酸的检测限（LOD）和定量限（LOQ）分别为7和22 nM，相对标准偏差（RSD）<10%。与文献中报道的用于生物硫醇分析的方法相比，本方法的优点是简单、快速和灵敏，而不需要耗时的分离和繁琐的预浓缩过程。此外，我们还发现，裸银纳米粒子可以直接用作基质的MALDI-TOF MS分析的硫药物，而无需添加额外的质子源。
A strategy is presented for the analysis of sulfur drugs and biothiols using silver nanoparticles (AgNPs) capped with different functional groups as the matrix and affinity probes in surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF MS) and atmospheric pressure-matrix assisted laser desorption/ionization ion trap mass spectrometry (AP-MALDI-ITMS). Biothiols adsorbed on the surface of AgNPs through covalent bonding were subjected to ultraviolet (UV) radiation that enabled desorption and ionization due to the excellent photochemical property of NPs. The proposed method has been successfully applied for the determination of cysteine and homocysteine in human urine samples using an internal standard. The limit of detection (LOD) and limit of quantification (LOQ) for cysteine and homocysteine in urine sample are 7 and 22 nM, respectively, with a relative standard deviation (RSD) of <10%. The advantages of the present method compared with the methods reported in the literature for biothiol analysis are simplicity, rapidity and sensitivity without the need for time-consuming separation and tedious preconcentration processes. Additionally, we also found that the bare AgNPs can be directly used as the matrix in MALDI-TOF MS for the analysis of sulfur drugs without the addition of an extra proton source.