Nitrogen-Doped Carbon Dots as A New Substrate for Sensitive Glucose Determination.
Nitrogen-Doped Carbon Dots as A New Substrate for Sensitive Glucose Determination.
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DOI：
10.3390/s16050630
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发表时间：
2016-05-04
期刊：
影响因子：
--
通讯作者：
Jia X
中科院分区：
文献类型：
--
作者：
Ji H;Zhou F;Gu J;Shu C;Xi K;Jia X
介绍了氮掺杂碳点作为一种新型的酶固定化底物在电化学检测中的应用。用聚丙烯酰胺一步就能很容易地合成氮掺杂碳点。利用壳聚糖上的氨基，通过氨基-羧基反应将葡萄糖氧化酶固定在氮掺杂碳点修饰碳玻璃电极上。氮掺杂碳点上氮诱导的电荷离域可以增强氧还原的电催化活性。特定的氨基羧基反应提供了GOx在电极上的强而稳定的固定。该传感器在1 ~ 12 mM的浓度范围内对血清样品中葡萄糖的存在作出有效响应，检测限为0.25 mM。该传感器具有良好的重现性和稳定性，在生理条件下对葡萄糖的测定具有很高的选择性。这些结果表明，n掺杂量子点是构建电化学生物传感器的一种新的候选材料。
Nitrogen-doped carbon dots are introduced as a novel substrate suitable for enzyme immobilization in electrochemical detection metods. Nitrogen-doped carbon dots are easily synthesised from polyacrylamide in just one step. With the help of the amino group on chitosan, glucose oxidase is immobilized on nitrogen-doped carbon dots-modified carbon glassy electrodes by amino-carboxyl reactions. The nitrogen-induced charge delocalization at nitrogen-doped carbon dots can enhance the electrocatalytic activity toward the reduction of O2. The specific amino-carboxyl reaction provides strong and stable immobilization of GOx on electrodes. The developed biosensor responds efficiently to the presence of glucose in serum samples over the concentration range from 1 to 12 mM with a detection limit of 0.25 mM. This novel biosensor has good reproducibility and stability, and is highly selective for glucose determination under physiological conditions. These results indicate that N-doped quantum dots represent a novel candidate material for the construction of electrochemical biosensors.