Promoter and transcription start site of human and rabbit butyrylcholinesterase genes.
Promoter and transcription start site of human and rabbit butyrylcholinesterase genes.
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人和兔丁酰胆碱酯酶基因的启动子和转录起始位点。
DOI：
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发表时间：
1994
影响因子：
4.8
通讯作者：
O. Lockridge
中科院分区：
文献类型：
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作者：
O. Jbilo;J. Toutant;K. P. Vatsis;A. Chatonnet;O. Lockridge
人类和家兔的丁基胆碱酯酶（BCHE）基因的5'-非翻译区有两个千碱基片段。除了人类BCHE中存在的333碱基对（bp） Alu重复序列外，这些序列具有广泛的同一性。通过引物延伸、mRNA 5′端扩增和RNase保护技术，在两个基因中发现了一个单一的转录起始位点。人和家兔BCHE基因的Cap位点位于严格同源的位置。在人BCHE中，转录起始位点位于翻译起始位点Met-28上游157 bp处。这两个启动子的潜在调控元件包括一个AP1位点和多个拓扑异构酶、Oct-1和PEA-3位点。BCHE报告基因构建的瞬时表达表明，人BCHE 5'侧区的194 bp片段和兔BCHE的570 bp片段足以促进HeLa细胞中氯霉素乙酰转移酶的活性。没有找到一致的TATA和CAAT盒子。然而，转录起始位点周围的序列与发育调节基因中其他TATA-less启动子中的启动元件具有同源性。
Two kilobase segments of the 5'-untranslated regions of the human and rabbit butyrylcholinesterase (BCHE) genes were characterized. The sequences shared extensive identity except for a 333-base pair (bp) Alu repeat present only in human BCHE. One single transcription start site was found in both genes with the techniques of primer extension, amplification of the 5'-end of mRNA, and RNase protection. Cap sites in human and rabbit BCHE genes were found in strictly homologous positions. In human BCHE, the transcription start site was found 157 bp upstream of Met-28, the translation start site. Potential regulatory elements in both promoters included one AP1 site and multiple sites for topoisomerase, Oct-1 and PEA-3. Transient expression of BCHE-reporter gene constructs showed that a 194-bp fragment of the 5'-flanking region of human BCHE and a 570-bp fragment of rabbit BCHE were sufficient for promoting chloramphenicol acetyltransferase activity in HeLa cells. No consensus TATA and CAAT boxes were found. However, the sequence around the transcription start site exhibited homology with initiator elements found in other TATA-less promoters in developmentally regulated genes.