Conserved Roles of Fibroblast Growth Factor Receptor 2 Signaling in the Regulation of Inner Cell Mass Development in Bovine Blastocysts
Conserved Roles of Fibroblast Growth Factor Receptor 2 Signaling in the Regulation of Inner Cell Mass Development in Bovine Blastocysts
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DOI：
10.1002/mrd.22646
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发表时间：
2016-06-01
影响因子：
2.5
通讯作者：
Kawahara, Manabu
中科院分区：
文献类型：
--
作者：
Akizawa, Hiroki;Nagatomo, Hiroaki;Kawahara, Manabu
在植入前哺乳动物发育过程中的一个常见过程是胚泡形成，其利用通过成纤维细胞生长因子受体2（FGFR 2）的信号传导，然而FGFR 2信号传导影响牛胚胎植入前发育的机制仍然不完全清楚。在这里，我们使用RNA干扰，调查在体外发育，卵裂球凋亡的频率，和发育标记基因的mRNA表达在FGF受体2敲低（FGFR 2-KD）牛胚胎。FGFR 2 mRNA的减少不影响植入前发育或凋亡卵裂球的频率，但确实增强了囊胚内细胞团的增殖（P< 0.05）-这与使用药理学方法（用泛FGFR阻断剂PD 173074处理）报告的牛胚胎表型不同，但与先前使用小鼠胚胎获得的结果一致。此外，外胚层标记基因NANOG和原始内胚层标记基因GATA 6的表达保持不变，而另一个原始内胚层标记基因HNF 4A的表达在FGFR 2-KD胚胎中显著降低。因此，FGFR 2信号传导似乎与牛囊胚形成期间内细胞团发育和增殖的调节相关。
A common process during preimplantation mammalian development is blastocyst formation, which utilizes signaling through fibroblast growth factor receptor 2 (FGFR2), yet the mechanisms through which FGFR2 signaling affect preimplantation development in bovine embryos remain incompletely understood. Here, we used RNA-interference to investigate the in vitro development, the frequency of blastomere apoptosis, and the mRNA expression of developmental marker genes in FGF receptor 2-knockdown (FGFR2-KD) bovine embryos. A reduction in FGFR2 mRNA did not affect preimplantation development or the frequency of apoptotic blastomeres, but did enhanced proliferation of the inner cell mass in blastocysts (P< 0.05) -which differs from the phenotype reported for bovine embryos using a pharmacological approach (treatment with the pan-FGFR blocker PD173074), but agrees with previous results obtained using mouse embryos. Moreover, the expression of an epiblast marker gene, NANOG, and a primitive endoderm marker gene, GATA6, remained unchanged, whereas the expression of another primitive endoderm marker gene, HNF4A, was significantly reduced in FGFR2-KD embryos. Therefore, FGFR2 signaling appears to be associated with the regulation of inner cell mass development and proliferation during blastocyst formation in cattle.