Reduction of the magnetic signal from unbound magnetic markers for magnetic immunoassay without bound/free separation
Reduction of the magnetic signal from unbound magnetic markers for magnetic immunoassay without bound/free separation
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DOI：
10.1016/j.physc.2007.04.284
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发表时间：
2007-10-01
影响因子：
1.7
通讯作者：
Enpuku, K.
中科院分区：
文献类型：
--
作者：
Tsukamoto, A.;Kuma, H.;Enpuku, K.
我们研究了添加磁屏蔽和施加补偿场来降低非束缚自由标记体的磁信号的效果。当初始免疫分析系统使用时，没有额外的屏蔽盒，液体标记物经Fe3O4重量归一化后的信号强度为干燥标记物的1/2700。通过安装额外的屏蔽箱，降低系数提高到1/10,000。我们成功地利用补偿场进一步降低了液体标记的磁信号。施加- 18 nT的补偿场，得到的自由标记的磁信号为1.4 m Phi(0)，接近系统噪声级。在SQUID周围的非常局部区域进行场补偿，足以减少来自自由标记的磁信号。(C) 2007 Elsevier B.V.版权所有
We investigated the effect of adding magnetic shielding and applying of a compensation field to reduce the magnetic signal from unbound free markers. When the initial immunoassay system was used without the additional shielding box, the signal intensity of the liquid markers normalized by the weight of the Fe3O4 was 1/2700 of the dried markers. By installing the additional shielding box, the reduction factor was improved to 1/10,000. We successfully reduced the magnetic signal of the liquid marker further by applying a compensation field. The magnetic signal of the free marker obtained by applying a compensation field of - 18 nT was 1.4 m Phi(0), which was close to the system noise level. Field compensation at the very local area just around the SQUID is sufficient for reducing of the magnetic signal from the free marker. (C) 2007 Elsevier B.V. All rights reserved.