Light Modulation of Cellular cAMP by a Small Bacterial Photoactivated Adenylyl Cyclase, bPAC, of the Soil Bacterium Beggiatoa
Light Modulation of Cellular cAMP by a Small Bacterial Photoactivated Adenylyl Cyclase, bPAC, of the Soil Bacterium Beggiatoa
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DOI：
10.1074/jbc.m110.185496
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发表时间：
2011-01-14
影响因子：
4.8
通讯作者：
Hegemann, Peter
中科院分区：
文献类型：
--
作者：
Stierl, Manuela;Stumpf, Patrick;Hegemann, Peter
最近在光遗传学中通道视紫红质的成功也引起了人们对直接被光激活的酶的兴趣。我们在Beggiatoa细菌的基因组中发现了一个编码腺苷酸环化酶的DNA序列，该酶与BLUF（使用FAD的蓝光受体）型光传感器结构域直接相连。在大肠杆菌和非洲爪蟾卵母细胞中，这种光激活腺苷酸环化酶（bPAC）的环化酶活性在黑暗中较低，但在光照下增加300倍。这种酶活性在20秒内与红移blf光中间体并行热衰减。bPAC在锥体神经元中表达良好，并与环核苷酸门控通道结合，引起有效的光诱导去极化。在果蝇中枢神经系统中，bPAC介导光依赖性cAMP的增加和自由运动动物的行为改变。bPAC似乎是神经细胞和组织中cAMP光调制以及研究活体动物cAMP依赖过程的完美光遗传学工具。
The recent success of channelrhodopsin in optogenetics has also caused increasing interest in enzymes that are directly activated by light. We have identified in the genome of the bacterium Beggiatoa a DNA sequence encoding an adenylyl cyclase directly linked to a BLUF (blue light receptor using FAD) type light sensor domain. In Escherichia coli and Xenopus oocytes, this photoactivated adenylyl cyclase (bPAC) showed cyclase activity that is low in darkness but increased 300-fold in the light. This enzymatic activity decays thermally within 20 s in parallel with the red-shifted BLUF photointermediate. bPAC is well expressed in pyramidal neurons and, in combination with cyclic nucleotide gated channels, causes efficient light-induced depolarization. In the Drosophila central nervous system, bPAC mediates light-dependent cAMP increase and behavioral changes in freely moving animals. bPAC seems a perfect optogenetic tool for light modulation of cAMP in neuronal cells and tissues and for studying cAMP-dependent processes in live animals.