Comparison of three preparatory methods for detection of bacteremia by MALDI-TOF mass spectrometry
Comparison of three preparatory methods for detection of bacteremia by MALDI-TOF mass spectrometry
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DOI：
10.1016/j.diagmicrobio.2012.01.010
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发表时间：
2012-05-01
影响因子：
2.9
通讯作者：
Patel, Robin
中科院分区：
文献类型：
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作者：
Saffert, Ryan T.;Cunningham, Scott A.;Patel, Robin
我们评价了在基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析前处理阳性血培养瓶肉汤的3种制备方法-差速离心、10%十二烷基硫酸钠（SDS）和Sepsityper试剂盒。初步评价使用的属和种水平临界值分别为1.700-1.999和>= 2.000。通过差速离心处理最初的79个血瓶培养物，得到51个（65%）属和34个（43%）种水平的正确鉴定。用10% SDS和Sepsityper同时处理101个不同的血液瓶。两种方法分别对77瓶（76%）和49瓶（49%）和54瓶（54%）瓶进行了属级鉴定。调整属（1.500-1.699）和种（>= 1.700）的评分截止标准提高了识别百分比，特别是对于种水平的识别（P < 0.05）。(C)2012 Elsevier Inc. All rights reserved.
We evaluated 3 preparatory methods for processing positive blood culture bottle broths prior to matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis-differential centrifugation, 10% sodium dodecyl sulfate (SDS), and the Sepsityper kit. Initial evaluation used genus and species level cutoff scores of 1.700-1.999 and >= 2.000, respectively. Processing of an initial 79 blood bottle cultures by differential centrifugation yielded correct identifications of 51 (65%) to the genus and 34(43%) to the species levels. One hundred and one different blood bottles were simultaneously processed with 10% SDS and Sepsityper. Both yielded genus-level identifications; for 77 (76%), and the 2 yielded species-level identifications for 49 (49%) and 54 (54%) of bottles, respectively. Adjustment of the score cutoff criteria for genus (1.500-1.699) and species (>= 1.700) improved identification percentages, particularly for species-level identifications (P < 0.05). (C) 2012 Elsevier Inc. All rights reserved.