The localization of lectin binding sites on photoreceptor outer segments and pigment epithelium of dystrophic retinas.
The localization of lectin binding sites on photoreceptor outer segments and pigment epithelium of dystrophic retinas.
复制标题
凝集素结合位点在营养不良性视网膜的光感受器外节和色素上皮上的定位。
DOI：
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发表时间：
1980
影响因子：
4.4
通讯作者：
J. Wood
中科院分区：
文献类型：
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作者：
B. Mclaughlin;J. Wood
用小麦胚芽凝集素（WGA）、蓖麻凝集素（RCA）、透镜凝集素（LCA）和伴刀豆球蛋白A（Con A）的过氧化物酶结合凝集素对14 ~ 16日龄皇家外科学院（RCS）大鼠及其遗传控制（RCS-rdy+）的色素上皮（PE）和光感受器外节（OS）膜上的含碳水化合物大分子进行了定位。所有凝集素染色的感光细胞内节和完整的OS的正常（RCS-rdy+）和营养不良（RCS）视网膜的质膜。在正常视网膜中，所有凝集素也染色脱落的OS的质膜，并且WGA染色一些椎间盘内膜。相比之下，WGA，RCA和Con A不标记营养不良视网膜中的OS碎片膜，但LCA标记其中的一些。在正常和异位视网膜中，WGA均匀地标记PE微绒毛的近端和远端膜表面，而RCA主要标记远端区域。Con A标记正常和营养不良的PE微绒毛稀疏，LCA染色RCS rdy+视网膜中的PE微绒毛比RCS视网膜中的PE微绒毛更强烈。在正常和营养不良的视网膜中的凝集素标记之间的主要差异是在OS碎片上存在LCA染色，并且在这些膜上不存在任何其他凝集素染色。其他差异是营养不良PE微绒毛膜上的稀疏LCA染色与正常和正常视网膜的OS椎间盘内膜上存在WGA染色。这些差异可能反映了OS膜和PE微绒毛上某些细胞表面糖的可及性或组成的变化，这可能与RCS视网膜中吞噬作用的降低速率有关。
Carbohydrate-containing macromolecules on pigment epithelium (PE) and photoreceptor outer segment (OS) membranes of 14 to 16-day-old Royal College of Surgeons (RCS) rats and their genetic control (RCS-rdy+) have been localized with peroxidase-conjugated lectins from wheat germ agglutinin (WGA), Ricinus communis (RCA), Lens culinaris (LCA), and concanavalin A (Con A). All lectins stain the plasma membranes of photoreceptor inner segments and intact OSs of normal (RCS-rdy+) and dystrophic (RCS) retinas. In the normal retinas, all lectins stain also the plasma membranes of shed OSs, and WGA stains some intradisc membranes. In contrast, WGA, RCA, and Con A do not label the OS debris membranes in dystrophic retinas, but LCA labels some of them. In both normal and dystophic retinas, WGA uniformly labels both proximal and distal membrane surfaces of PE mcirovilli, whereas RCA labels primarily the distal regions. Con A labels both normal and dystrophic PE microvilli sparsely, and LCA stains the PE microvilli in RCS-rdy+ retinas more intensely than those in the RCS retinas. The major differences between the lectin labeling in normal and dystrophic retinas are the presence of LCA staining on OS debris and the absence of any other lectin staining on these membranes. Other differences are the sparse LCA staining on dystrophic PE microvillous membranes vs. the normal and the presence of WGA staining on OS intradisc membranes of normal retinas. These differences may reflect changes in the accessibility or composition of certain cell surface sugars on OS membranes and PE microvilli which may be related to the diminished rate of phagocytosis in RCS retinas.