Identification of the Set of Genes, Including Nonannotated morA, under the Direct Control of ModE in Escherichia coli
Identification of the Set of Genes, Including Nonannotated morA, under the Direct Control of ModE in Escherichia coli
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DOI：
10.1128/jb.00304-13
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发表时间：
2013-10-01
影响因子：
3.2
通讯作者：
Yamamoto, Kaneyoshi
中科院分区：
文献类型：
--
作者：
Kurata, Tatsuaki;Katayama, Akira;Yamamoto, Kaneyoshi
ModE是一种能感应氨基酸的转录调节因子，它控制着大肠杆菌中与内环境稳定相关的基因的表达。ModE通过结合β-内酰胺酶而被激活，并且充当激活剂和阻遏物。通过配体的基因组系统进化指数富集（SELEX）筛选和启动子报告基因分析，我们已经确定了总共9个操纵子，包括迄今为止确定的modA，moaA，dmsA，和napF操纵子，其中6个被激活的ModE和3个被抑制。另外，新鉴定了两个启动子，分别位于yghW和toryY的反义链上，并指导新基因的转录，称为莫拉和morB。莫拉基因编码一个短肽，莫拉，具有一个不寻常的起始密码子。令人惊讶的是，过表达莫拉5'非翻译区对E. coli K-12。
ModE is the molybdate-sensing transcription regulator that controls the expression of genes related to molybdate homeostasis in Escherichia coli. ModE is activated by binding molybdate and acts as both an activator and a repressor. By genomic systematic evolution of ligands by exponential enrichment (SELEX) screening and promoter reporter assays, we have identified a total of nine operons, including the hitherto identified modA, moaA, dmsA, and napF operons, of which six were activated by ModE and three were repressed. In addition, two promoters were newly identified and direct transcription of novel genes, referred to as morA and morB, located on antisense strands of yghW and torY, respectively. The morA gene encodes a short peptide, MorA, with an unusual initiation codon. Surprisingly, overexpression of the morA 5' untranslated region exhibited an inhibitory influence on colony formation of E. coli K-12.